The podocyte-specific knockout of palladin in mice with a 129 genetic background affects podocyte morphology and the expression of palladin interacting proteins
Artelt, Nadine; Ritter, Alina; Leitermann, Linda; Kliewe, Felix; Schlüter, Rabea; Simm, Stefan; van den Brandt, Jens; Endlich, Karlhans; Endlich, Nicole (2021)
Artelt, Nadine; Ritter, Alina; Leitermann, Linda; Kliewe, Felix; Schlüter, Rabea...
PloS One 16 (12), e0260878.
DOI: 10.1371/journal.pone.0260878
Open Access Peer Reviewed
Proper and size selective blood filtration in the kidney depends on an intact morphology of podocyte foot processes. Effacement of interdigitating podocyte foot processes in the glomeruli causes a leaky filtration barrier resulting in proteinuria followed by the development of chronic kidney diseases. Since the function of the filtration barrier is depending on a proper actin cytoskeleton, we studied the role of the important actin-binding protein palladin for podocyte morphology. Podocyte-specific palladin knockout mice on a C57BL/6 genetic background (PodoPalldBL/6-/-) were back crossed to a 129 genetic background (PodoPalld129-/-) which is known to be more sensitive to kidney damage. Then we analyzed the morphological changes of glomeruli and podocytes as well as the expression of the palladin-binding partners Pdlim2, Lasp-1, Amotl1, ezrin and VASP in 6 and 12 months old mice. PodoPalld129-/- mice in 6 and 12 months showed a marked dilatation of the glomerular tuft and a reduced expression of the mesangial marker protein integrin α8 compared to controls of the same age. Furthermore, ultrastructural analysis showed significantly more podocytes with morphological deviations like an enlarged sub-podocyte space and regions with close contact to parietal epithelial cells. Moreover, PodoPalld129-/- of both age showed a severe effacement of podocyte foot processes, a significantly reduced expression of pLasp-1 and Pdlim2, and significantly reduced mRNA expression of Pdlim2 and VASP, three palladin-interacting proteins. Taken together, the results show that palladin is essential for proper podocyte morphology in mice with a 129 background.
Circulation of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli of Pandemic Sequence Types 131, 648, and 410 Among Hospitalized Patients, Caregivers, and the Community in Rwanda
Eger, Elias; Heiden, Stefan; Korolew, Katja; Bayingana, Claude; Ndoli, Jules; Sendegeya, Augustin; Gahutu, Jean; Kurz, Mathis; Mockenhaupt, Frank; Müller, Julia; Simm, Stefan; Schaufler, Katharina (2021)
Eger, Elias; Heiden, Stefan; Korolew, Katja; Bayingana, Claude; Ndoli, Jules...
Frontiers in Microbiology 12, 662575.
DOI: 10.3389/fmicb.2021.662575
Open Access Peer Reviewed
Multi-drug resistant (MDR), gram-negative Enterobacteriaceae, such as Escherichia coli (E. coli) limit therapeutic options and increase morbidity, mortality, and treatment costs worldwide. They pose a serious burden on healthcare systems, especially in developing countries like Rwanda. Several studies have shown the effects caused by the global spread of extended-spectrum beta-lactamase (ESBL)-producing E. coli. However, limited data is available on transmission dynamics of these pathogens and the mobile elements they carry in the context of clinical and community locations in Sub-Saharan Africa. Here, we examined 120 ESBL-producing E. coli strains from patients hospitalized in the University Teaching Hospital of Butare (Rwanda), their attending caregivers as well as associated community members and livestock. Based on whole-genome analysis, the genetic diversification and phylogenetics were assessed. Moreover, the content of carried plasmids was characterized and investigated for putative transmission among strains, and for their potential role as drivers for the spread of antibiotic resistance. We show that among the 30 different sequence types (ST) detected were the pandemic clonal lineages ST131, ST648 and ST410, which combine high-level antimicrobial resistance with virulence. In addition to the frequently found resistance genes bla CTX-M-15 , tet(34), and aph(6)-Id, we identified csg genes, which are required for curli fiber synthesis and thus biofilm formation. Numerous strains harbored multiple virulence-associated genes (VAGs) including pap (P fimbriae adhesion cluster), fim (type I fimbriae) and chu (Chu heme uptake system). Furthermore, we found phylogenetic relationships among strains from patients and their caregivers or related community members and animals, which indicates transmission of pathogens. Also, we demonstrated the presence and potential transfer of identical/similar ESBL-plasmids in different strains from the Rwandan setting and when compared to an external plasmid. This study highlights the circulation of clinically relevant, pathogenic ESBL-producing E. coli among patients, caregivers and the community in Rwanda. Combining antimicrobial resistance with virulence in addition to the putative exchange of mobile genetic elements among bacterial pathogens poses a significant risk around the world.
Identification and Regulation of Tomato Serine/Arginine-Rich Proteins Under High Temperatures
Rosenkranz, Remus; Bachiri, Samia; Vraggalas, Stavros; Keller, Mario; Simm, Stefan; Schleiff, Enrico; Fragkostefanakis, Sotirios (2021)
Rosenkranz, Remus; Bachiri, Samia; Vraggalas, Stavros; Keller, Mario; Simm, Stefan...
Frontiers in Plant Science 12, 645689.
DOI: 10.3389/fpls.2021.645689
Open Access Peer Reviewed
Alternative splicing is an important mechanism for the regulation of gene expression in eukaryotes during development, cell differentiation or stress response. Alterations in the splicing profiles of genes under high temperatures that cause heat stress (HS) can impact the maintenance of cellular homeostasis and thermotolerance. Consequently, information on factors involved in HS-sensitive alternative splicing is required to formulate the principles of HS response. Serine/arginine-rich (SR) proteins have a central role in alternative splicing. We aimed for the identification and characterization of SR-coding genes in tomato (Solanum lycopersicum), a plant extensively used in HS studies. We identified 17 canonical SR and two SR-like genes. Several SR-coding genes show differential expression and altered splicing profiles in different organs as well as in response to HS. The transcriptional induction of five SR and one SR-like genes is partially dependent on the master regulator of HS response, HS transcription factor HsfA1a. Cis-elements in the promoters of these SR genes were predicted, which can be putatively recognized by HS-induced transcription factors. Further, transiently expressed SRs show reduced or steady-state protein levels in response to HS. Thus, the levels of SRs under HS are regulated by changes in transcription, alternative splicing and protein stability. We propose that the accumulation or reduction of SRs under HS can impact temperature-sensitive alternative splicing.
Promoting health by traveling: Mindfulness and travel medicine
Kohls, Niko (2020)
Health Promotion & Physical Activity, 2020, 4 (13), 62-64.
Peer Reviewed
Mobilität, Erreichbarkeit, Raum: (Selbst-)kritische Perspektiven aus Wissenschaft und Praxis
Appel, Alexandra; Scheiner, Joachim; Wilde, Mathias (2020)
Das Recht auf Bildung im permanenten Krisenzustand. Zur globalen Bildungssituation
Lohrenscheit , Claudia (2020)
Reihe: Aus Politik und Zeitgeschichte 51/2020, 165-174.
Open Access
Das Recht auf Bildung im permanenten Krisenzustand. Zur globalen Bildungssituation
Lohrenscheit , Claudia (2020)
Bundeszentrale für politische Bildung (Redaktion APuZ -Aus Politik und Zeigeschichte). Corona. Pandemie und Krise, Frankfurt/M. 2021, 165-174.
Open Access
3.7.2 Schwangere/ Frauen ab 18 Jahren / Frauen als Angehörige.
Vogt, Irmgard; Bischof, Gallus; Hannak-Zeltner, Renate; Hößelbarth, Susann; Jückstock, Julia; Hoffmann, Sabine (2020)
Vogt, Irmgard; Bischof, Gallus; Hannak-Zeltner, Renate; Hößelbarth, Susann...
Vogt, I., Bischof, G.; Hannak-Zeltner, R.; Hößelbarth, S.; Jückstock, J. & Hoffmann, S. (2020). 3.7.2 Schwangere/ Frauen ab 18 Jahren / Frauen als Angehörige. In: Deutsche Gesellschaft für Psychiatrie und Psychotherapie, Psychosomatik und Nervenheilkunde (DGPPN) & Deutsche Gesellschaft für Suchtforschung und Suchttherapie e.V. (DG-SUCHT) (Hrsg.). S3 Leitlinie „Screening, Diagnose und Behandlung alkoholbezogener Störungen”. Version Dezember 2020 (S. 270-312). Verfügbar unter: https://www.awmf.org/leitlinien/detail /ll/076-001.html. [27.04.2022], 270-312.
Open Access
Das gesundheitsökonomische Potential des Einsatzes von Placebos in der Behandlung von ADHS
Hamberger, Jens; Hinterberger , Thilo ; Loew, T.; Meißner, Karin; Weimer, K. (2020)
e-Poster beim Kongress der Deutschen Gesellschaft für Psychiatrie und Psychotherapie, Psychosomatik und Nervenheilkunde (DGPPN), 26.11.2020, Online-Kongress.
Räumliche Navigation durch richtungsgebundene Stereofonie
Markert, Michael (2020)
Dissertation, Bauhaus-Universität Weimar, 2020.
DOI: 10.25643/bauhaus-universitaet.4303
Open Access Peer Reviewed
Von Nemesis und Narziss: Lektionen, die COVID bei Vorhaben - und der Ethik - globaler Gesundheitsförderung und Biosicherheit erteilen kann
DiEuliis, D.; Kohls, Niko; Giordano, J. (2020)
In M. Woesler & H. M. Sass (Eds.), Medizin und Ethik in Zeiten von Corona (pp. 323 - 327), 323-327.
Effects of the Gastein Healing Gallery on physical functioning, activity and participation – results of qualitative interviews of 14 patients with rheumatoid arthritis (RA), ankylosing spondylitis (AS) or fibromyalgia syndrome (FMS)
Offenbaecher, M.; Zerhoch, Tamara; Nöfer, Eberhard; Kohls, Niko; Toussaint, L.; Sirios , F. ; Untner, J.; Hirsch, J. K. (2020)
Offenbaecher, M.; Zerhoch, Tamara; Nöfer, Eberhard; Kohls, Niko; Toussaint, L....
Eingereicht Österreichischer Kongress für Rheumatologie 2020..
Peer Reviewed
Depression and Pain in Patients with Rheumatic and Musculoskeletal Disease: Are Positive Psychological Characteristics Protective?
Altier, H.; Offenbaecher, M.; van der Zee-Neuen, A.; Toussaint, L.; Kohls, Niko; Hanshans, Christian ; Sirios , F. ; Untner, J.; Hölzl, B.; Gaisberger, M.; Hirsch, J. K. (2020)
Altier, H.; Offenbaecher, M.; van der Zee-Neuen, A.; Toussaint, L.; Kohls, Niko...
Eingereicht Österreichischer Kongress für Rheumatologie 2020..
DOI: 10.13140/RG.2.2.23795.81443
Peer Reviewed
The perceived benefit and harm of mild radon hyperthermia, rehabilitation and medications from the point of view of a large cohort of patients with ankylosing spondylitis (AS) attending the Gastein Healing Gallery
Offenbaecher, M.; Hirsch, J. K.; Sirios , F. ; van der Zee-Neuen, A.; Scharmga, A.; Lunzer, R.; Herold, Manfred; Untner, J.; Kohls, Niko; Hölzl, B.; Gaisberger, M.; Toussaint, L. (2020)
Offenbaecher, M.; Hirsch, J. K.; Sirios , F. ; van der Zee-Neuen, A.; Scharmga, A....
Eingereicht Österreichischer Kongress für Rheumatologie 2020..
Peer Reviewed
The perceived benefit and harm of active exercise, physical therapy and modalities of a large cohort of patients with ankylosing spondylitis (AS) attending the Gastein Healing Gallery
Offenbacher, M.; Hirsch, J. K.; Sirios , F. ; van der Zee-Neuen, A.; Scharmga, A.; Lunzer, R.; Untner, J.; Kohls, Niko; Hölzl, B.; Gaisberger, M.; Toussaint, L. (2020)
Offenbacher, M.; Hirsch, J. K.; Sirios , F. ; van der Zee-Neuen, A.; Scharmga, A....
Eingereicht Österreichischer Kongress für Rheumatologie 2020.
Peer Reviewed
Effects of the Gastein Healing Gallery on physical functioning, activity and participation – results of qualitative interviews of 14 patients with rheumatoid arthritis (RA), ankylosing spondylitis (AS) or fibromyalgia syndrome (FMS)
Offenbaecher, M.; Zerhoch, Tamara; Nöfer, Eberhard; Toussaint, L.; Kohls, Niko; Sirios , F. ; Untner, J.; Hirsch, J. K. (2020)
Offenbaecher, M.; Zerhoch, Tamara; Nöfer, Eberhard; Toussaint, L.; Kohls, Niko...
J Miner Stoffwechs Muskuloskelet Erkrank: 27:141.
Peer Reviewed
The perceived benefit and harm of mild radon hyperthermia, rehabilitation and medications from the point of view of a large cohort of patients with ankylosing spondylitis (AS)
Offenbaecher, M.; Hirsch, J. K.; Sirios , F. ; van der Zee-Neuen, A.; Scharmga, A.; Lunzer, R.; Untner, J.; Kohls, Niko; Hölzl, B.; Gaisberger, M.; Toussaint, L. (2020)
Offenbaecher, M.; Hirsch, J. K.; Sirios , F. ; van der Zee-Neuen, A.; Scharmga, A....
attending the Gastein Healing Gallery. J Miner Stoffwechs Muskuloskelet Erkrank;27:145.
Peer Reviewed
The perceived benefit and harm of active exercise, physical therapy and modalities of a large cohort of patients with ankylosing spondylitis (AS) attending the Gastein Healing Gallery
Offenbaecher, M.; Hirsch, J. K.; Sirios , F. ; van der Zee-Neuen, A.; Scharmga, A.; Lunzer, R.; Untner, J.; Kohls, Niko; Hölzl, B.; Gaisberger, M.; Toussaint, L. (2020)
Offenbaecher, M.; Hirsch, J. K.; Sirios , F. ; van der Zee-Neuen, A.; Scharmga, A....
J Miner Stoffwechs Muskuloskelet Erkrank: 27:142.
Peer Reviewed
Effects of the Gastein Healing Gallery on medication use and physical and mental health – results of qualitative interviews of 14 patients with rheumatoid arthritis (RA), ankylosing spondylitis (AS) or fibromyalgia syndrome (FMS)
Offenbaecher, M.; Zerhoch, Tamara; Nöfer, Eberhard; Kohls, Niko; Toussaint, L.; Sirios , F. ; van der Zee-Neuen, A.; Untner, J.; Hirsch, J. K. (2020)
Offenbaecher, M.; Zerhoch, Tamara; Nöfer, Eberhard; Kohls, Niko; Toussaint, L....
Suppl2:S96.
Peer Reviewed
Spatial Resolution-Independent CNN-based Person Detection in Agricultural Image Data
Strutz, Tilo; Leipnitz, Alexander; Jokisch, Oliver (2020)
5th Int. Conf. on Interactive Collaborative Robotics, ICR.
Peer Reviewed
Advanced object detectors based on Convolutional Neural Networks (CNNs) offer high detection rates for many application scenarios but only within their respective training, validation and test data. Recent studies show that such methods provide a limited generalization ability for unknown data, even for small image modifications including a limited scale invariance. Reliable person detection with aerial robots (Unmanned Aerial Vehicles, UAVs) is an essential task to fulfill high security requirements or to support robot control, communication, and human-robot interaction. Particularly in an agricultural context persons need to be detected from a long distance and a high altitude to allow the UAV an adequate and timely response. While UAVs are able to produce high resolution images that enable the detection of persons from a longer distance, typical CNN input layer sizes are comparably low. The inevitable scaling of images to match the input-layer size can lead to a further reduction in person sizes. We investigate the reliability of different YOLOv3 architectures for person detection in regard to those input-scaling effects. The popular VisDrone data set with its varying image resolutions and relatively small depiction of humans is used as well as high resolution UAV images from an agricultural data set. To overcome the scaling problem, an algorithm is presented for segmenting high resolution images in overlapping tiles that match the input-layer size. The number and overlap of the tiles are dynamically determined based on the image resolution. It is shown that the detection rate of very small persons in high resolution images can be improved using this tiling approach.
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