Neuroethical Address of Neuroscience and Technology to Support and Foster Integration of Complementary Approaches Within Healthcare Systems: A Paradigmatic Thesis; Abstr Intl Neuroethics Society; 2013.
Hadley, T.; Kohls, Niko; Plischke , Herbert ; Giordano, J. (2013)
A Paradigmatic Thesis; Abstr Intl Neuroethics Society; 2013.
Peer Reviewed
Die Rückgabe eines Buches des protestantischen Gelehrten Wilhelm Wundt aus dem Besitz des enteigneten jüdischen Apothekers Leopold Scheyer an seine Erben - Teil II.
Andert, K.; Kohls, Niko (2013)
Bibliotheksdienst, 47(5), 308 - 314.
Rasch-Analyse des Freiburger Fragebogens zur Achtsamkeit
Sauer, S.; Strobl, C.; Walach, H.; Kohls, Niko (2013)
Diagnostica, 59(2), 86-99..
Peer Reviewed
Exceptional experiences, mindfulness, spiritual practice, and mental distress
Kohls, Niko; Silver, C. F.; Hood, R. W.; Walach, H. (2013)
8th annual congress of the International Society for Complementary Medicine Research (ISCMR), London, 11-13.3.2013 .
Peer Reviewed
The protein translocation systems in plants - composition and variability on the example of Solanum lycopersicum
Paul, Puneet; Simm, Stefan; Blaumeiser, Andreas; Scharf, Klaus-Dieter; Fragkostefanakis, Sotirios; Mirus, Oliver; Schleiff, Enrico (2013)
Paul, Puneet; Simm, Stefan; Blaumeiser, Andreas; Scharf, Klaus-Dieter...
BMC genomics 14, 189.
DOI: 10.1186/1471-2164-14-189
Open Access Peer Reviewed
BACKGROUND: Protein translocation across membranes is a central process in all cells. In the past decades the molecular composition of the translocation systems in the membranes of the endoplasmic reticulum, peroxisomes, mitochondria and chloroplasts have been established based on the analysis of model organisms. Today, these results have to be transferred to other plant species. We bioinformatically determined the inventory of putative translocation factors in tomato (Solanum lycopersicum) by orthologue search and domain architecture analyses. In addition, we investigated the diversity of such systems by comparing our findings to the model organisms Saccharomyces cerevisiae, Arabidopsis thaliana and 12 other plant species. RESULTS: The literature search end up in a total of 130 translocation components in yeast and A. thaliana, which are either experimentally confirmed or homologous to experimentally confirmed factors. From our bioinformatic analysis (PGAP and OrthoMCL), we identified (co-)orthologues in plants, which in combination yielded 148 and 143 orthologues in A. thaliana and S. lycopersicum, respectively. Interestingly, we traced 82% overlap in findings from both approaches though we did not find any orthologues for 27% of the factors by either procedure. In turn, 29% of the factors displayed the presence of more than one (co-)orthologue in tomato. Moreover, our analysis revealed that the genomic composition of the translocation machineries in the bryophyte Physcomitrella patens resemble more to higher plants than to single celled green algae. The monocots (Z. mays and O. sativa) follow more or less a similar conservation pattern for encoding the translocon components. In contrast, a diverse pattern was observed in different eudicots. CONCLUSIONS: The orthologue search shows in most cases a clear conservation of components of the translocation pathways/machineries. Only the Get-dependent integration of tail-anchored proteins seems to be distinct. Further, the complexity of the translocation pathway in terms of existing orthologues seems to vary among plant species. This might be the consequence of palaeoploidisation during evolution in plants; lineage specific whole genome duplications in Arabidopsis thaliana and triplications in Solanum lycopersicum.
Impact of the solid angle of light sources upon the suppression of melatonin in adults – a feasibility study
Novotny, P.; Schwarz, M.; Kohls, Niko; Plischke , Herbert (2013)
6. Deutscher AAL-Kongress 2013, Berlin, 22.01.2013 - 23.01.2013.
Peer Reviewed
Fixierungen aus Fürsorge - Die Anwendung freiheitsentziehender Maßnahmen bei Pflegebedürftigen
Berzlanovich, A.; Kirsch, S.; Herold-Majumdar, A.; Randzio, O; Schöpfer, J.; Keil, W; Kohls, Niko (2013)
Berzlanovich, A.; Kirsch, S.; Herold-Majumdar, A.; Randzio, O; Schöpfer, J.; Keil, W...
In Deutsche Gesellschaft für Gynäkologie und Geburtshilfe und Hessisches Sozialministerium (Ed.), Gewalt - Ärztliches Praxishandbuch (pp. 193 - 200). Berlin: Kramarz. , 193-200.
Decision technologies: Practical considerations, ethico-legal implications, and the need for dialectical evaluation
Rossi, J.; Novotny, P.; Plischke , Herbert ; Kohls, Niko; Giordano, J. (2013)
J Ethics Engineer Biol Med .
Peer Reviewed
Conscious Presence and Self Control as a measure of situational awareness in soldiers—A validation study
Büssing, Arndt; Walach, H.; Kohls, Niko; Zimmermann, Fred; Trousselard, M. (2013)
International Journal of Mental Health Systems, 7(1), 1..
Peer Reviewed
Characterization of a single-chain variable fragment recognizing a linear epitope of Aß: A biotechnical tool for studies on Alzheimer's disease?
Dornieden, Silke; Müller-Schiffmann, A.; Sticht, H.; Cinar, Yeliz ; Wördehoff, M.; Korth , C.; Funke, Susanne A.; Willbold, Dieter (2013)
Dornieden, Silke; Müller-Schiffmann, A.; Sticht, H.; Cinar, Yeliz ; Wördehoff, M....
Lawrence, Kan. PLoS 2013 , 8 | e59820.
DOI: 10.1371/journal.pone.0059820
Peer Reviewed
The amyloid-β oligomer count in cerebrospinal fluid is a biomarker for Alzheimer's disease
Wang-Dietrich , Lei ; Funke, Susanne A.; Kühlbach , Katja ; Wang , Kun; Besmehn, Astrid ; Willbold , S.; Cinar, Yeliz ; Bannach , Oliver; Birkmann, Eva; Willbold, Dieter (2013)
Wang-Dietrich , Lei ; Funke, Susanne A.; Kühlbach , Katja ; Wang , Kun...
National Library of Medicine
National Center for Biotechnology Information, 34.
DOI: 10.3233/JAD-122047.
Open Access Peer Reviewed
Mobilität als soziale Praxis: Ein handlungstheoretischer Blick auf Bewegung
Wilde, Mathias (2013)
Mobilitäten und Immobilitäten: Menschen, Ideen, Dinge, Kulturen, Kapital 142, 35-48.
Peer Reviewed
Mobilität und Alter: Anregungen für eine Mobilitätsforschung aus akteurszentrierter Perspektive
Wilde, Mathias (2013)
Verkehrszeichen für Mobilität und Umwelt 29 (2), 13-23.
Defining the core proteome of the chloroplast envelope membranes
Simm, Stefan; Papasotiriou, Dimitrios; Ibrahim, Mohamed; Leisegang, Matthias; Müller, Bernd; Schorge, Tobias; Karas, Michael; Mirus, Oliver; Sommer, Maik; Schleiff, Enrico (2013)
Simm, Stefan; Papasotiriou, Dimitrios; Ibrahim, Mohamed; Leisegang, Matthias...
Frontiers in Plant Science 4, 11.
DOI: 10.3389/fpls.2013.00011
Open Access Peer Reviewed
High-throughput protein localization studies require multiple strategies. Mass spectrometric analysis of defined cellular fractions is one of the complementary approaches to a diverse array of cell biological methods. In recent years, the protein content of different cellular (sub-)compartments was approached. Despite of all the efforts made, the analysis of membrane fractions remains difficult, in that the dissection of the proteomes of the envelope membranes of chloroplasts or mitochondria is often not reliable because sample purity is not always warranted. Moreover, proteomic studies are often restricted to single (model) species, and therefore limited in respect to differential individual evolution. In this study we analyzed the chloroplast envelope proteomes of different plant species, namely, the individual proteomes of inner and outer envelope (OE) membrane of Pisum sativum and the mixed envelope proteomes of Arabidopsis thaliana and Medicago sativa. The analysis of all three species yielded 341 identified proteins in total, 247 of them being unique. 39 proteins were genuine envelope proteins found in at least two species. Based on this and previous envelope studies we defined the core envelope proteome of chloroplasts. Comparing the general overlap of the available six independent studies (including ours) revealed only a number of 27 envelope proteins. Depending on the stringency of applied selection criteria we found 231 envelope proteins, while less stringent criteria increases this number to 649 putative envelope proteins. Based on the latter we provide a map of the outer and inner envelope core proteome, which includes many yet uncharacterized proteins predicted to be involved in transport, signaling, and response. Furthermore, a foundation for the functional characterization of yet unidentified functions of the inner and OE for further analyses is provided.
40S ribosome biogenesis co-factors are essential for gametophyte and embryo development
Missbach, Sandra; Weis, Benjamin; Martin, Roman; Simm, Stefan; Bohnsack, Markus; Schleiff, Enrico (2013)
Missbach, Sandra; Weis, Benjamin; Martin, Roman; Simm, Stefan; Bohnsack, Markus...
PloS One 8 (1), e54084.
DOI: 10.1371/journal.pone.0054084
Open Access Peer Reviewed
Ribosome biogenesis is well described in Saccharomyces cerevisiae. In contrast only very little information is available on this pathway in plants. This study presents the characterization of five putative protein co-factors of ribosome biogenesis in Arabidopsis thaliana, namely Rrp5, Pwp2, Nob1, Enp1 and Noc4. The characterization of the proteins in respect to localization, enzymatic activity and association with pre-ribosomal complexes is shown. Additionally, analyses of T-DNA insertion mutants aimed to reveal an involvement of the plant co-factors in ribosome biogenesis. The investigated proteins localize mainly to the nucleolus or the nucleus, and atEnp1 and atNob1 co-migrate with 40S pre-ribosomal complexes. The analysis of T-DNA insertion lines revealed that all proteins are essential in Arabidopsis thaliana and mutant plants show alterations of rRNA intermediate abundance already in the heterozygous state. The most significant alteration was observed in the NOB1 T-DNA insertion line where the P-A3 fragment, a 23S-like rRNA precursor, accumulated. The transmission of the T-DNA through the male and female gametophyte was strongly inhibited indicating a high importance of ribosome co-factor genes in the haploid stages of plant development. Additionally impaired embryogenesis was observed in some mutant plant lines. All results support an involvement of the analyzed proteins in ribosome biogenesis but differences in rRNA processing, gametophyte and embryo development suggested an alternative regulation in plants.
Freiheitsentziehende Maßnahmen in der Pflege – Wo ist die Grenze zwischen Fürsorge und Übergriff?
Berzlanovich, A.; Kirsch, S.; Schöpfer, J.; Keil, W; Kohls, Niko (2012)
MMW - Fortschritte der Medizin, 154(20), 53-55, 53-55.
Gerichtliche Genehmigungspraxis und pflegewissenschaftlicher Ansatz bei freiheitsentziehenden Maßnahmen - ein Widerspruch?
Berzlanovich, A.; Kirsch, L.; Kohls, Niko (2012)
Betreuungsrechtliche Praxis (3), 95 - 99., 95 - 99.
Peer Reviewed
Structural analysis of the pyroglutamate-modified isoform of the Alzheimer's disease-related amyloid-β using NMR spectroscopy
Sun, Na; Hartmann, Rudolf ; Lecher, Justin ; Stoldt , Matthias ; Funke, Susanne A.; Gremer, Lothar; Ludwig , Hans-Henning ; Demuth, Hans-Ulrich; Kleinschmidt , Martin ; Willbold, Dieter (2012)
Sun, Na; Hartmann, Rudolf ; Lecher, Justin ; Stoldt , Matthias ; Funke, Susanne A....
J Pept Sci
2012 Nov;18(11):691-5 2012, 18.
DOI: 10.1002/psc.2456
Open Access Peer Reviewed
Mirror image phage display--generating stable therapeutically and diagnostically active peptides with biotechnological means
Sun, Na; Funke, Susanne A.; Willbold, Dieter (2012)
J Biotechnol 161, 2.
DOI: 10.1016/j.jbiotec.2012.05.019
Open Access Peer Reviewed
Quality of life in patients with fibromyalgia: validation and psychometric properties of the German Quality of Life Scale (QOLS-G)
Offenbächer, M.; Sauer, S.; Kohls, Niko; Waltz, M.; Schoeps, P. (2012)
Rheumatology International, 32(10), 3243-3252, 3243-3252.
Peer Reviewed