The association of late-acting snoRNPs with human pre-ribosomal complexes requires the RNA helicase DDX21
Sloan, Katherine; Leisegang, Matthias; Doebele, Carmen; Ramírez, Ana; Simm, Stefan; Safferthal, Charlotta; Kretschmer, Jens; Schorge, Tobias; Markoutsa, Stavroula; Haag, Sara; Karas, Michael; Ebersberger, Ingo; Schleiff, Enrico; Watkins, Nicholas; Bohnsack, Markus (2015)
Sloan, Katherine; Leisegang, Matthias; Doebele, Carmen; Ramírez, Ana; Simm, Stefan...
Nucleic Acids Research 43 (1), 553–564.
DOI: 10.1093/nar/gku1291
Open Access Peer Reviewed
Translation fidelity and efficiency require multiple ribosomal (r)RNA modifications that are mostly mediated by small nucleolar (sno)RNPs during ribosome production. Overlapping basepairing of snoRNAs with pre-rRNAs often necessitates sequential and efficient association and dissociation of the snoRNPs, however, how such hierarchy is established has remained unknown so far. Here, we identify several late-acting snoRNAs that bind pre-40S particles in human cells and show that their association and function in pre-40S complexes is regulated by the RNA helicase DDX21. We map DDX21 crosslinking sites on pre-rRNAs and show their overlap with the basepairing sites of the affected snoRNAs. While DDX21 activity is required for recruitment of the late-acting snoRNAs SNORD56 and SNORD68, earlier snoRNAs are not affected by DDX21 depletion. Together, these observations provide an understanding of the timing and ordered hierarchy of snoRNP action in pre-40S maturation and reveal a novel mode of regulation of snoRNP function by an RNA helicase in human cells.
Detection and Quantitation of Amyloid-β Aggregates in Body Fluids may be Suitable for Early Diagnosis of Alzheimer’s Disease
Funke, Susanne A.; Birkmann, Eva; Willbold, Dieter (2014)
Advances in Alzheimer's Research
2.
DOI: 10.2174/9781608058525114020013
Phonorama: Mobile Spatial Navigation By Directional Stereophony
Markert, Michael; Geelhaar, Jens (2014)
MobileHCI '14: Proceedings of the 16th International Conference on Human-Computer Interaction with Mobile Devices and Services 16, 609-611.
DOI: 10.1145/2628363.2645700
Peer Reviewed
Resilienz, Zufriedenheit und kooperative Zusammenarbeit im Unternehmensalltag
Tamdjidi, C.; Kohls, Niko (2014)
Gesundheitswesen, 76(08/09), A196. doi: 10.1055/s-0034-1387046.
DOI: https://doi.org:10.1055/s-0034-1387046
Peer Reviewed
Rezension zu Foljanty, Lena, Recht oder Gesetz. Juristische Identität und Autorität in den Naturrechtsdebatten der Nachkriegszeit
Buchholz-Schuster, Eckardt (2014)
Zeitschrift der Savigny-Stiftung für Rechtsgeschichte, Germanistische Abteilung 2014/ Bd. 131, 560 ff..
DOI: 10.7767/zrgga-2014-0147
Modellbildung und Simulation von photovoltaisch angetriebenen Wärmepumpensystemen
Schaub, Michael (2014)
Masterarbeit, Hochschule Biberach, 2014.
Open Access
A pre-ribosomal RNA interaction network involving snoRNAs and the Rok1 helicase
Martin, Roman; Hackert, Philipp; Ruprecht, Maike; Simm, Stefan; Brüning, Lukas; Mirus, Oliver; Sloan, Katherine; Kudla, Grzegorz; Schleiff, Enrico; Bohnsack, Markus (2014)
Martin, Roman; Hackert, Philipp; Ruprecht, Maike; Simm, Stefan; Brüning, Lukas...
RNA (New York, N.Y.) 20 (8), 1173–1182.
DOI: 10.1261/rna.044669.114
Open Access Peer Reviewed
Ribosome biogenesis in yeast requires 75 small nucleolar RNAs (snoRNAs) and a myriad of cofactors for processing, modification, and folding of the ribosomal RNAs (rRNAs). For the 19 RNA helicases implicated in ribosome synthesis, their sites of action and molecular functions have largely remained unknown. Here, we have used UV cross-linking and analysis of cDNA (CRAC) to reveal the pre-rRNA binding sites of the RNA helicase Rok1, which is involved in early small subunit biogenesis. Several contact sites were identified in the 18S rRNA sequence, which interestingly all cluster in the "foot" region of the small ribosomal subunit. These include a major binding site in the eukaryotic expansion segment ES6, where Rok1 is required for release of the snR30 snoRNA. Rok1 directly contacts snR30 and other snoRNAs required for pre-rRNA processing. Using cross-linking, ligation and sequencing of hybrids (CLASH) we identified several novel pre-rRNA base-pairing sites for the snoRNAs snR30, snR10, U3, and U14, which cluster in the expansion segments of the 18S rRNA. Our data suggest that these snoRNAs bridge interactions between the expansion segments, thereby forming an extensive interaction network that likely promotes pre-rRNA maturation and folding in early pre-ribosomal complexes and establishes long-range rRNA interactions during ribosome synthesis.
Individual differences in self-attributed mindfulness levels are related to the experience of time and cognitive self-control
Wittmann, M.; Peter, J.; Gutina, O.; Otten, S.; Kohls, Niko; Meißner, Karin (2014)
Personality and Individual Differences, 64(0), 41-45. , 41-45.
Peer Reviewed
Freiheitsberaubung aus Fürsorge?! – Die Anwendung freiheitsentziehender Maßnahmen in der Pflege
Berzlanovich, A.; Kirsch, S.; Herold-Majumdar, A.; Randzio, O; Kohls, Niko (2014)
In T. Gaertner, B. Gansweid, H. Gerber, F. Schwegler, & U. Heine (Eds.), Die Pflegeversicherung: Handbuch zur Begutachtung, Qualitätsprüfung, Beratung und Fortbildung (3 ed., pp. 462-470). Berlin: Walter de Gruyter., 462-470.
Wie wollen wir pflegen und gepflegt werden? Versorgung pflegebedürftiger Angehöriger in der Zukunft
Offenbacher, M.; Herold-Majumdar, Astrid; Frauenhofer, Andreas; Thomas, Lisa; Roletscheck, Heike; Kohls, Niko; Bührlen, Brigitte (2014)
Offenbacher, M.; Herold-Majumdar, Astrid; Frauenhofer, Andreas; Thomas, Lisa...
Lage: Jacobs Verlag 2014..
Mindfulness in German Schools (MISCHO): A Specifically Tailored Training Program: Concept, Implementation and Empirical Results
Kaltwasser, V.; Sauer, S.; Kohls, Niko (2014)
In S. Schmidt & H. Walach (Eds.), Meditation – Neuroscientific Approaches and Philosophical Implications (Vol. 2, pp. 381-404): Springer International Publishing., 381-404.
The Hidden Appeal. Anthropomorphic, Biomorphic and Narrative Aspects of Visual Perception in Spatial Arts, Architecture & Design
Heinrich, Michael (2014)
Vortrag und Proceedings, 5th Annual International Conference on Visual and Performing Arts, Athens Institute for Education and Research, 2014.
Opposite Drug Prescription and Cost Trajectories following Integrative and Conventional Care for Pain - A Case-Control Study
Sundberg, Tobias; Petzold, M.; Kohls, Niko; Falkenberg, T. (2014)
PLoS ONE, 9(5). .
Peer Reviewed
Efficient and Effective Testing of Automotive Software Product Lines
Cmyrev , A. ; Reißing, Ralf (2014)
ESIT 2014, April 2014, Krabi. .
Einflussfaktoren auf Studienzufriedenheit sowie Prävention psychischer Gefährdung bei Medizinstudierenden - Ein Vergleich zwischen LMU München und Uni Witten/Herdecke
Jurkat, H.; Christina, U.; V., Anke; Janosch,, R.; Sauer, S.; Ott, U.; Büssing, Arndt; Kohls, Niko (2014)
Jurkat, H.; Christina, U.; V., Anke; Janosch,, R.; Sauer, S.; Ott, U.; Büssing, Arndt...
Paper presented at the Deutscher Kongress für Psychosomatische Medizin und Psychotherapie Berlin, 26 – 29.3.2014..
Peer Reviewed
Mind-Body-Ansätze
Esch, Tobias; Offenbächer, M.; Kohls, Niko (2014)
In P. Angerer, J. Glaser, H. Gündel, P. Henningsen, C. Lahmann, S. Letzel & D. Nowak (Eds.), Psychische und psychosomatische Gesundheit in der Arbeit (pp. 261 - 268). Landsberg: Ecomed., 261-268.
Form & Function Toolkit: Printed Electronics for Unconventional Interface
Geelhaar, Jens; Markert, Michael; Deich, Johannes; Schied, Martin (2014)
Proceedings of the 8th International Conference on Tangible, Embedded and Embodied Interaction 2014, 365-368.
DOI: 10.1145/2540930.2567900
Peer Reviewed
The evolution of the ribosome biogenesis pathway from a yeast perspective
Ebersberger, Ingo; Simm, Stefan; Leisegang, Matthias; Schmitzberger, Peter; Mirus, Oliver; von Haeseler, Arndt; Bohnsack, Markus; Schleiff, Enrico (2014)
Ebersberger, Ingo; Simm, Stefan; Leisegang, Matthias; Schmitzberger, Peter...
Nucleic Acids Research 42 (3), 1509–1523.
DOI: 10.1093/nar/gkt1137
Open Access Peer Reviewed
Ribosome biogenesis is fundamental for cellular life, but surprisingly little is known about the underlying pathway. In eukaryotes a comprehensive collection of experimentally verified ribosome biogenesis factors (RBFs) exists only for Saccharomyces cerevisiae. Far less is known for other fungi, animals or plants, and insights are even more limited for archaea. Starting from 255 yeast RBFs, we integrated ortholog searches, domain architecture comparisons and, in part, manual curation to investigate the inventories of RBF candidates in 261 eukaryotes, 26 archaea and 57 bacteria. The resulting phylogenetic profiles reveal the evolutionary ancestry of the yeast pathway. The oldest core comprising 20 RBF lineages dates back to the last universal common ancestor, while the youngest 20 factors are confined to the Saccharomycotina. On this basis, we outline similarities and differences of ribosome biogenesis across contemporary species. Archaea, so far a rather uncharted domain, possess 38 well-supported RBF candidates of which some are known to form functional sub-complexes in yeast. This provides initial evidence that ribosome biogenesis in eukaryotes and archaea follows similar principles. Within eukaryotes, RBF repertoires vary considerably. A comparison of yeast and human reveals that lineage-specific adaptation via RBF exclusion and addition characterizes the evolution of this ancient pathway.
The D-amino acid peptide D3 reduces amyloid fibril boosted HIV-1 infectivity
Widera , Marek ; Klein, Antonia Nicole ; Cinar, Yeliz ; Funke, Susanne A.; Willbold, Dieter; Schaal , Heiner (2014)
Widera , Marek ; Klein, Antonia Nicole ; Cinar, Yeliz ; Funke, Susanne A....
AIDS Research and Therapy 11, 1.
DOI: 10.1186/1742-6405-11-1
Open Access Peer Reviewed
Amyloid aggregation inhibitory mechanism of arginine-rich D-peptides
Olubiyi , O. O. ; Frenzel , Oliver ; Bartnik , Dirk ; Glück , J. M. ; Brener, Oleksandr; Nagel-Steger, Luitgard; Funke, Susanne A.; Willbold, Dieter; Strodel , B. (2014)
Olubiyi , O. O. ; Frenzel , Oliver ; Bartnik , Dirk ; Glück , J. M. ; Brener, Oleksandr...
National Library of Medicine
National Center for Biotechnology Information, 1448-57.
DOI: 10.2174/0929867321666131129122247
Open Access Peer Reviewed