Sensorik zur Regelung von reaktionsschnellen Wärmeübergabesystemen
Schmitt, Lukas; Schaub, Michael; Kriegel, Martin (2021)
TGA-Kongress, 28.-29.01.2021 in Berlin / Online.
DOI: 10.13140/RG.2.2.34007.34726
Open Access
Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus
Coelho, Vitor Lima; Fontenele de Brito, Tarcísio; Alexandre de Abreu Brito, Ingrid; Arruda Cardoso, Maira; Berni, Mateus Antonio; Araujo, Helena Maria Marcolla; Sammeth, Michael; Pane, Attilio (2021)
Coelho, Vitor Lima; Fontenele de Brito, Tarcísio; Alexandre de Abreu Brito, Ingrid...
Scientific Reports 2021 (11), 1918.
DOI: 10.1038/s41598-021-81387-1
Open Access Peer Reviewed
Rhodnius prolixus is a Triatominae insect species and a primary vector of Chagas disease. The genome of R. prolixus has been recently sequenced and partially assembled, but few transcriptome analyses have been performed to date. In this study, we describe the stage-specific transcriptomes obtained from previtellogenic stages of oogenesis and from mature eggs. By analyzing ~ 228 million paired-end RNA-Seq reads, we significantly improved the current genome annotations for 9206 genes. We provide extended 5' and 3' UTRs, complete Open Reading Frames, and alternative transcript variants. Strikingly, using a combination of genome-guided and de novo transcriptome assembly we found more than two thousand novel genes, thus increasing the number of genes in R. prolixus from 15,738 to 17,864. We used the improved transcriptome to investigate stage-specific gene expression profiles during R. prolixus oogenesis. Our data reveal that 11,127 genes are expressed in the early previtellogenic stage of oogenesis and their transcripts are deposited in the developing egg including key factors regulating germline development, genome integrity, and the maternal-zygotic transition. In addition, GO term analyses show that transcripts encoding components of the steroid hormone receptor pathway, cytoskeleton, and intracellular signaling are abundant in the mature eggs, where they likely control early embryonic development upon fertilization. Our results significantly improve the R. prolixus genome and transcriptome and provide novel insight into oogenesis and early embryogenesis in this medically relevant insect.
Positive Psychological Factors and Impairment in Rheumatic and Musculoskeletal Disease: Do Psychopathology and Sleep Quality Explain the Linkage?
Hirsch, J. K.; Altier, H.; Offenbächer, M.; Toussaint, L.; Kohls, Niko; Sirios , F. (2021)
Hirsch, J. K.; Altier, H.; Offenbächer, M.; Toussaint, L.; Kohls, Niko...
Arthritis Care & Research, n/a(n/a).
DOI: 10.1002/acr.24440
Peer Reviewed
Effectiveness of Progressive Muscle Relaxation, Deep Breathing, and Guided Imagery
Toussaint, L.; Nguyen, Q. A.; Roettger, C.; Dixon, K.; Offenbächer, M.; Kohls, Niko; Hirsch, Jameson; Sirois, F. (2021)
Toussaint, L.; Nguyen, Q. A.; Roettger, C.; Dixon, K.; Offenbächer, M.; Kohls, Niko...
in Promoting Psychological and Physiological States of Relaxation. Evidence-based Complementary and Alternative Medicine, 2021, 5924040. doi:10.1155/2021/5924040.
Peer Reviewed
Hygrothermal characterization of a fictitious homogenized porous material to describe multiphase heat and moisture transport in massive historic walls
Bottino-Leone, Dario; Larcher, Marco; Troi, Alexandra; Grunewald, John (2021)
Construction and Building Materials 266.
DOI: 10.1016/j.conbuildmat.2020.121497
Peer Reviewed
Consumer Experience durch Einsatz von Mixed Reality in Einkaufsumgebungen
Zagel, Christian (2021)
Multisensorik im stationären Handel - Grundlagen und Praxis der kundenzentrierten Filialgestaltung 2021, 221-235.
DOI: 10.1007/978-3-658-31273-2
Dynamic Capabilities: Celebrating the Plurality of Understandings of the Concept
Wenzel, M.; Rauch, M.; Abiodun Adegbile, A.; Bogodistov, Y.; Cénophat, S.; Hartmann, Michael; Wagner, D.; Wohlgemuth , V. (2021)
Wenzel, M.; Rauch, M.; Abiodun Adegbile, A.; Bogodistov, Y.; Cénophat, S....
Dynamic Capabilities and Relationships: Discourses, Concepts, and Reflections 2021, 5-41.
DOI: 10.1007/978-3-030-83182-0_1
Open Access
Menschenrechte verteidigen. Perspektivwechsel für Solidarität und Inklusion
Lohrenscheit , Claudia (2021)
Achour, Sabine/Gill, Thomas (Hg.): Politische Bildung und Flucht – ein Paradigmenwechsel?!, Frankfurt/Main 2021, 57-65.
ZukunftsDesign - Ein Studiengang der Regionalentwicklung
Zagel, Christian; Hasenkopf , Hauke ; Hoffmann, Mark (2021)
Hochschule Coburg .
Open Access
Positive Psychological Factors and Impairment in Rheumatic and Musculoskeletal Disease: Do Psychopathology and Sleep Quality Explain the Linkage?
Hirsch, J. K.; Altier, H.; Offenbächer, M.; Toussaint, L.; Kohls, Niko; Sirois, F. M. (2021)
Hirsch, J. K.; Altier, H.; Offenbächer, M.; Toussaint, L.; Kohls, Niko...
Arthritis Care & Research 2021 73 (1), 55–64.
DOI: 10.1002/acr.24440
Peer Reviewed
OBJECTIVE
Pain is not the major determinant of quality of life in fibromyalgia - results from a retrospective "real world" data analysis of fibromyalgia patients
Offenbächer, M.; Kohls, Niko; Ewert, T.; Sigl, C.; Hieblinger, R.; Toussaint, L.; Sirois, F. M.; Hirsch, J. K.; Vallejo, M. A.; Kramer, S.; Rivera, J.; Stucki, G.; Schelling, J.; Winkelmann, A. (2021)
Offenbächer, M.; Kohls, Niko; Ewert, T.; Sigl, C.; Hieblinger, R.; Toussaint, L....
Rheumatology International 2021.
DOI: 10.1007/s00296-020-04702-5
Peer Reviewed
OBJECTIVE
Sportstadt Coburg. Ideen für die Regionalentwicklung
Holtorf, Christian (2021)
Ergebnisse interdisziplinärer Lehre Band 2 2021.
Open Access
The podocyte-specific knockout of palladin in mice with a 129 genetic background affects podocyte morphology and the expression of palladin interacting proteins
Artelt, Nadine; Ritter, Alina; Leitermann, Linda; Kliewe, Felix; Schlüter, Rabea; Simm, Stefan; van den Brandt, Jens; Endlich, Karlhans; Endlich, Nicole (2021)
Artelt, Nadine; Ritter, Alina; Leitermann, Linda; Kliewe, Felix; Schlüter, Rabea...
PloS One 16 (12), e0260878.
DOI: 10.1371/journal.pone.0260878
Open Access Peer Reviewed
Proper and size selective blood filtration in the kidney depends on an intact morphology of podocyte foot processes. Effacement of interdigitating podocyte foot processes in the glomeruli causes a leaky filtration barrier resulting in proteinuria followed by the development of chronic kidney diseases. Since the function of the filtration barrier is depending on a proper actin cytoskeleton, we studied the role of the important actin-binding protein palladin for podocyte morphology. Podocyte-specific palladin knockout mice on a C57BL/6 genetic background (PodoPalldBL/6-/-) were back crossed to a 129 genetic background (PodoPalld129-/-) which is known to be more sensitive to kidney damage. Then we analyzed the morphological changes of glomeruli and podocytes as well as the expression of the palladin-binding partners Pdlim2, Lasp-1, Amotl1, ezrin and VASP in 6 and 12 months old mice. PodoPalld129-/- mice in 6 and 12 months showed a marked dilatation of the glomerular tuft and a reduced expression of the mesangial marker protein integrin α8 compared to controls of the same age. Furthermore, ultrastructural analysis showed significantly more podocytes with morphological deviations like an enlarged sub-podocyte space and regions with close contact to parietal epithelial cells. Moreover, PodoPalld129-/- of both age showed a severe effacement of podocyte foot processes, a significantly reduced expression of pLasp-1 and Pdlim2, and significantly reduced mRNA expression of Pdlim2 and VASP, three palladin-interacting proteins. Taken together, the results show that palladin is essential for proper podocyte morphology in mice with a 129 background.
Circulation of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli of Pandemic Sequence Types 131, 648, and 410 Among Hospitalized Patients, Caregivers, and the Community in Rwanda
Eger, Elias; Heiden, Stefan; Korolew, Katja; Bayingana, Claude; Ndoli, Jules; Sendegeya, Augustin; Gahutu, Jean; Kurz, Mathis; Mockenhaupt, Frank; Müller, Julia; Simm, Stefan; Schaufler, Katharina (2021)
Eger, Elias; Heiden, Stefan; Korolew, Katja; Bayingana, Claude; Ndoli, Jules...
Frontiers in Microbiology 12, 662575.
DOI: 10.3389/fmicb.2021.662575
Open Access Peer Reviewed
Multi-drug resistant (MDR), gram-negative Enterobacteriaceae, such as Escherichia coli (E. coli) limit therapeutic options and increase morbidity, mortality, and treatment costs worldwide. They pose a serious burden on healthcare systems, especially in developing countries like Rwanda. Several studies have shown the effects caused by the global spread of extended-spectrum beta-lactamase (ESBL)-producing E. coli. However, limited data is available on transmission dynamics of these pathogens and the mobile elements they carry in the context of clinical and community locations in Sub-Saharan Africa. Here, we examined 120 ESBL-producing E. coli strains from patients hospitalized in the University Teaching Hospital of Butare (Rwanda), their attending caregivers as well as associated community members and livestock. Based on whole-genome analysis, the genetic diversification and phylogenetics were assessed. Moreover, the content of carried plasmids was characterized and investigated for putative transmission among strains, and for their potential role as drivers for the spread of antibiotic resistance. We show that among the 30 different sequence types (ST) detected were the pandemic clonal lineages ST131, ST648 and ST410, which combine high-level antimicrobial resistance with virulence. In addition to the frequently found resistance genes bla CTX-M-15 , tet(34), and aph(6)-Id, we identified csg genes, which are required for curli fiber synthesis and thus biofilm formation. Numerous strains harbored multiple virulence-associated genes (VAGs) including pap (P fimbriae adhesion cluster), fim (type I fimbriae) and chu (Chu heme uptake system). Furthermore, we found phylogenetic relationships among strains from patients and their caregivers or related community members and animals, which indicates transmission of pathogens. Also, we demonstrated the presence and potential transfer of identical/similar ESBL-plasmids in different strains from the Rwandan setting and when compared to an external plasmid. This study highlights the circulation of clinically relevant, pathogenic ESBL-producing E. coli among patients, caregivers and the community in Rwanda. Combining antimicrobial resistance with virulence in addition to the putative exchange of mobile genetic elements among bacterial pathogens poses a significant risk around the world.
Identification and Regulation of Tomato Serine/Arginine-Rich Proteins Under High Temperatures
Rosenkranz, Remus; Bachiri, Samia; Vraggalas, Stavros; Keller, Mario; Simm, Stefan; Schleiff, Enrico; Fragkostefanakis, Sotirios (2021)
Rosenkranz, Remus; Bachiri, Samia; Vraggalas, Stavros; Keller, Mario; Simm, Stefan...
Frontiers in Plant Science 12, 645689.
DOI: 10.3389/fpls.2021.645689
Open Access Peer Reviewed
Alternative splicing is an important mechanism for the regulation of gene expression in eukaryotes during development, cell differentiation or stress response. Alterations in the splicing profiles of genes under high temperatures that cause heat stress (HS) can impact the maintenance of cellular homeostasis and thermotolerance. Consequently, information on factors involved in HS-sensitive alternative splicing is required to formulate the principles of HS response. Serine/arginine-rich (SR) proteins have a central role in alternative splicing. We aimed for the identification and characterization of SR-coding genes in tomato (Solanum lycopersicum), a plant extensively used in HS studies. We identified 17 canonical SR and two SR-like genes. Several SR-coding genes show differential expression and altered splicing profiles in different organs as well as in response to HS. The transcriptional induction of five SR and one SR-like genes is partially dependent on the master regulator of HS response, HS transcription factor HsfA1a. Cis-elements in the promoters of these SR genes were predicted, which can be putatively recognized by HS-induced transcription factors. Further, transiently expressed SRs show reduced or steady-state protein levels in response to HS. Thus, the levels of SRs under HS are regulated by changes in transcription, alternative splicing and protein stability. We propose that the accumulation or reduction of SRs under HS can impact temperature-sensitive alternative splicing.
Promoting health by traveling: Mindfulness and travel medicine
Kohls, Niko (2020)
Health Promotion & Physical Activity, 2020, 4 (13), 62-64.
Peer Reviewed
Mobilität, Erreichbarkeit, Raum: (Selbst-)kritische Perspektiven aus Wissenschaft und Praxis
Appel, Alexandra; Scheiner, Joachim; Wilde, Mathias (2020)
Das Recht auf Bildung im permanenten Krisenzustand. Zur globalen Bildungssituation
Lohrenscheit , Claudia (2020)
Reihe: Aus Politik und Zeitgeschichte 51/2020, 165-174.
Open Access
Das Recht auf Bildung im permanenten Krisenzustand. Zur globalen Bildungssituation
Lohrenscheit , Claudia (2020)
Bundeszentrale für politische Bildung (Redaktion APuZ -Aus Politik und Zeigeschichte). Corona. Pandemie und Krise, Frankfurt/M. 2021, 165-174.
Open Access
3.7.2 Schwangere/ Frauen ab 18 Jahren / Frauen als Angehörige.
Vogt, Irmgard; Bischof, Gallus; Hannak-Zeltner, Renate; Hößelbarth, Susann; Jückstock, Julia; Hoffmann, Sabine (2020)
Vogt, Irmgard; Bischof, Gallus; Hannak-Zeltner, Renate; Hößelbarth, Susann...
Vogt, I., Bischof, G.; Hannak-Zeltner, R.; Hößelbarth, S.; Jückstock, J. & Hoffmann, S. (2020). 3.7.2 Schwangere/ Frauen ab 18 Jahren / Frauen als Angehörige. In: Deutsche Gesellschaft für Psychiatrie und Psychotherapie, Psychosomatik und Nervenheilkunde (DGPPN) & Deutsche Gesellschaft für Suchtforschung und Suchttherapie e.V. (DG-SUCHT) (Hrsg.). S3 Leitlinie „Screening, Diagnose und Behandlung alkoholbezogener Störungen”. Version Dezember 2020 (S. 270-312). Verfügbar unter: https://www.awmf.org/leitlinien/detail /ll/076-001.html. [27.04.2022], 270-312.
Open Access