Positive Psychological Traits and Functional Impairment in Chronic Pain: Do Reduced Stress, Pain, and Fatigue Explain the Benefits?
Altier, H.; Offenbaecher, M.; Toussaint, L.; Kohls, Niko; Sirios , F. ; van der Zee-Neuen, A.; Hirsch, J. K. (2020)
Altier, H.; Offenbaecher, M.; Toussaint, L.; Kohls, Niko; Sirios , F. ...
Schmerz;34 Suppl.2: S91.
Peer Reviewed
Of Nemesis and Narcissus: Lessons COVID may provide for enterprises – and ethics – of global health promotion and biosecurity
DiEuliis, D.; Kohls, Niko; Giordano, J. (2020)
In M. Woesler & H. M. Sass (Eds.), Medicine and Ethics in Times of Corona (pp. 323 - 327), 323-327.
Educational needs of patients with rheumatic and musculoskeletal diseases attending a large health facility in Austria
Hirsch, Jameson; Toussaint, L.; Offenbächer, M.; Kohls, Niko; Hanshans, Christian ; Vallejo, M.; Rivera, J.; Sirios , F. ; Untner, J.; Hölzl, B.; Gaisberger, M.; Ndosi, M. (2020)
Hirsch, Jameson; Toussaint, L.; Offenbächer, M.; Kohls, Niko; Hanshans, Christian ...
Musculoskeletal Care, n/a(n/a). 2020 / 18 (3), 391 - 396.
DOI: 10.1002/msc.1474
Peer Reviewed
The Existence and Localization of Nuclear snoRNAs in Arabidopsis thaliana Revisited
Streit, Deniz; Shanmugam, Thiruvenkadam; Garbelyanski, Asen; Simm, Stefan; Schleiff, Enrico (2020)
Streit, Deniz; Shanmugam, Thiruvenkadam; Garbelyanski, Asen; Simm, Stefan...
Plants (Basel, Switzerland) 9 (8), 1016.
DOI: 10.3390/plants9081016
Open Access Peer Reviewed
Ribosome biogenesis is one cell function-defining process. It depends on efficient transcription of rDNAs in the nucleolus as well as on the cytosolic synthesis of ribosomal proteins. For newly transcribed rRNA modification and ribosomal protein assembly, so-called small nucleolar RNAs (snoRNAs) and ribosome biogenesis factors (RBFs) are required. For both, an inventory was established for model systems like yeast and humans. For plants, many assignments are based on predictions. Here, RNA deep sequencing after nuclei enrichment was combined with single molecule species detection by northern blot and in vivo fluorescence in situ hybridization (FISH)-based localization studies. In addition, the occurrence and abundance of selected snoRNAs in different tissues were determined. These approaches confirm the presence of most of the database-deposited snoRNAs in cell cultures, but some of them are localized in the cytosol rather than in the nucleus. Further, for the explored snoRNA examples, differences in their abundance in different tissues were observed, suggesting a tissue-specific function of some snoRNAs. Thus, based on prediction and experimental confirmation, many plant snoRNAs can be proposed, while it cannot be excluded that some of the proposed snoRNAs perform alternative functions than are involved in rRNA modification.
miRNAs involved in transcriptome remodeling during pollen development and heat stress response in Solanum lycopersicum
Keller, Mario; Schleiff, Enrico; Simm, Stefan (2020)
Scientific Reports 10 (1), 10694.
DOI: 10.1038/s41598-020-67833-6
Open Access Peer Reviewed
Cellular transitions during development and stress response depend on coordinated transcriptomic and proteomic alterations. Pollen is particular because its development is a complex process that includes meiotic and mitotic divisions which causes a high heat sensitivity of these cells. Development and stress response are accompanied by a reprogramming of the transcriptome, e.g. by post-transcriptional regulation via miRNAs. We identified known and potentially novel miRNAs in the transcriptome of developing and heat-stressed pollen of Solanum lycopersicum (tomato). The prediction of target mRNAs yielded an equal number of predicted target-sites in CDS and 3’UTR regions of target mRNAs. The result enabled the postulation of a possible link between miRNAs and a fine-tuning of transcription factor abundance during pollen development. miRNAs seem to play a role in the pollen heat stress response as well. We identified several heat stress transcription factors and heat shock proteins as putative targets of miRNAs in response to heat stress, thereby placing these miRNAs as important elements of thermotolerance. Moreover, for members of the AP2, SBP and ARF family members we could predict a miRNA-mediated regulation during development via the miR172, mir156 and mir160-family strengthening the current concept of a cross-connection between development and stress response in plants.
Freiheitsberaubung aus Fürsorge?! – Die Anwendung freiheitsentziehender Maßnahmen in der Pflege
Berzlanovich, A.; Kirsch, S.; Herold-Majumdar, A.; Kohls, Niko (2020)
In T. Gaertner, S. Knoblich, T. Muck, & M. Rieger (Eds.), Die Pflegeversicherung: Handbuch zur Begutachtung, Qualitätsprüfung, Beratung und Fortbildung (Vierte überarbeitete Auflage ed., pp. 637-646)., 637-646.
Depression and Pain in Patients with Rheumatic and Musculoskeletal Disease: Are Positive Psychological Characteristics Protective?
Altier, H.; Offenbaecher, M.; van der Zee-Neuen, A.; Toussaint, L.; Kohls, Niko; Hanshans, Christian ; Sirios , F. ; Untner, J.; Hölzl, B.; Gaisberger, M.; Hirsch, J. K. (2020)
Altier, H.; Offenbaecher, M.; van der Zee-Neuen, A.; Toussaint, L.; Kohls, Niko...
J Miner Stoffwechs Muskuloskelet Erkrank 27:145.
Peer Reviewed
Transcriptional Basis for Differential Thermosensitivity of Seedlings of Various Tomato Genotypes
Hu, Yangjie; Fragkostefanakis, Sotirios; Schleiff, Enrico; Simm, Stefan (2020)
Genes 11 (6), 655.
DOI: 10.3390/genes11060655
Open Access Peer Reviewed
Transcriptional reprograming after the exposure of plants to elevated temperatures is a hallmark of stress response which is required for the manifestation of thermotolerance. Central transcription factors regulate the stress survival and recovery mechanisms and many of the core responses controlled by these factors are well described. In turn, pathways and specific genes contributing to variations in the thermotolerance capacity even among closely related plant genotypes are not well defined. A seedling-based assay was developed to directly compare the growth and transcriptome response to heat stress in four tomato genotypes with contrasting thermotolerance. The conserved and the genotype-specific alterations of mRNA abundance in response to heat stress were monitored after exposure to three different temperatures. The transcripts of the majority of genes behave similarly in all genotypes, including the majority of heat stress transcription factors and heat shock proteins, but also genes involved in photosynthesis and mitochondrial ATP production. In turn, genes involved in hormone and RNA-based regulation, such as auxin- and ethylene-related genes, or transcription factors like HsfA6b, show a differential regulation that associates with the thermotolerance pattern. Our results provide an inventory of genes likely involved in core and genotype-dependent heat stress response mechanisms with putative role in thermotolerance in tomato seedlings.
Effekte von Lebensstiländerungen bei arterieller Hypertonie in der Primärversorgung - ein systematisches Review
Sanftenberg, L.; Badermann, M.; Kohls, Niko; Weber, Annemarie; Schelling, J.; Sirios , F. ; Toussaint, L.; Hirsch, Jameson; Offenbächer, M. (2020)
Sanftenberg, L.; Badermann, M.; Kohls, Niko; Weber, Annemarie; Schelling, J....
Zeitschrift für Evidenz, Fortbildung und Qualität im Gesundheitswesen, 150-152, 12-19. , 12-19.
DOI: 10.1016/j.zefq.2020.03.004
Peer Reviewed
Self-compassion and health in cardiac disease: Psychopathology as a mediator
Byerley, S.M.; Hirsch, J. K.; Weber, Annemarie; Kleinecke, C.; Hall, B.; Offenbächer, M.; Toussaint, L.; Sirios , F. ; Vajda, C.; Schelling, J.; Kohls, Niko; Brachmann, J. (2020)
Byerley, S.M.; Hirsch, J. K.; Weber, Annemarie; Kleinecke, C.; Hall, B....
Presentation at 66th annual conference of the Southeastern Psychological Association 2020, New Orleans, LA..
Peer Reviewed
Self-compassion and health in cardiac disease: Psychopathology as a mediator
Byerley, S. M.; Hirsch, J. K.; Weber, Annemarie; Kleinecke, C.; Hall, B.; Offenbaecher, M.; Toussaint, L.; Sirois, F.; Vajda, C.; Schelling, J.; Kohls, Niko; Brachmann, J. (2020)
Byerley, S. M.; Hirsch, J. K.; Weber, Annemarie; Kleinecke, C.; Hall, B....
Presentation at 66th annual conference of the Southeastern Psychological Association 2020, New Orleans, LA..
Peer Reviewed
Obituary: Professor Peter Gründler (1940–2019)
Flechsig, Gerd-Uwe (2020)
Journal of Solid State Electrochemistry 24, 795–796.
DOI: 10.1007/s10008-020-04518-0
Open Access Peer Reviewed
Toc75-V/OEP80 is processed during translocation into chloroplasts, and the membrane-embedded form exposes its POTRA domain to the intermembrane space
Gross, Lucia; Spies, Nicole; Simm, Stefan; Schleiff, Enrico (2020)
FEBS open bio 10 (3), 444–454.
DOI: 10.1002/2211-5463.12791
Open Access Peer Reviewed
The insertion of membrane proteins requires proteinaceous complexes in the cytoplasm, the membrane, and the lumen of organelles. Most of the required complexes have been described, while the components for insertion of β-barrel-type proteins into the outer membrane of chloroplasts remain unknown. The same holds true for the signals required for the insertion of β-barrel-type proteins. At present, only the processing of Toc75-III, the β-barrel-type protein of the central chloroplast translocon with an atypical signal, has been explored in detail. However, it has been debated whether Toc75-V/ outer envelope protein 80 (OEP80), a second protein of the same family, contains a signal and undergoes processing. To substantiate the hypothesis that Toc75-V/OEP80 is processed as well, we reinvestigated the processing in a protoplast-based assay as well as in native membranes. Our results confirm the existence of a cleavable segment. By protease protection and pegylation, we observed intermembrane space localization of the soluble N-terminal domain. Thus, Toc75-V contains a cleavable N-terminal signal and exposes its polypeptide transport-associated domains to the intermembrane space of plastids, where it likely interacts with its substrates.
Detection of the level of DNA cross-linking with cisplatin by electrochemical quartz crystal microbalance
Galagedera, S. K. K.; Flechsig, Gerd-Uwe (2020)
Journal of Electroanalytical Chemistry 862, 113992.
DOI: 10.1016/j.jelechem.2020.113992
Peer Reviewed
Biosensors based on DNA self-assembled monolayers (DNA-SAMs) combined with electrochemical transducers have shown great potential to serve an important role in simple, accurate and inexpensive genetic analysis relevant to many fields. Recently we have reported on observing a significant change in the viscoelasticity of such DNA layers immobilized on gold electrodes, upon redox switching of certain metal complexes at a millisecond time scale using an electrochemical quartz crystal microbalance (EQCM). In this study we have performed chronoamperometry on single stranded and double stranded DNA/6-mercapto-1-hexanol (MCH) mixed SAMs on gold electrodes in the presence of hexaammine ruthenium(III) (RuHex) to monitor the DNA modification with cis-diammine dichloroplatinum(II) (cisplatin). Our EQCM studies confirmed that the redox response of electrostatically bound RuHex is capable of providing quantitative information regarding the extent of DNA cross-linking with cisplatin. We believe that this method can be used in studies to test the interactions of cancer chemotherapeutic medications like cisplatin with ss & dsDNA and ultimately be a new technology for rapid screening of such potential drug candidates that are able to penetrate the blood-brain barrier and interact with DNA in order to inhibit cancer cell growth of brain tumor tissue by forming DNA adducts.
Die deutschsprachige Version der Combat Exposure Scale (D-CES) – erste psychometrische Validierung. Wehrmedizinische Monatsschrift.
Zimmermann, Fred; Walach, H.; Schubmann, R.; Kohls, Niko (2020)
Wehrmedizinische Monatsschrift, 64(2), 58-65. 2020 / 64 (2), 58-65.
Peer Reviewed
Benefits of gratitude and self-compassion for sleep in chronic pain: Mediating effects of stress, anxiety, and depression
Altier, H.; Toussaint, L.; Kohls, Niko; Hanshans, Christian ; Sirios , F. ; Untner, J.; Hölzl, B.; Gaisberger, M.; Offenbaecher, M.; Hirsch, J. K. (2020)
Altier, H.; Toussaint, L.; Kohls, Niko; Hanshans, Christian ; Sirios , F. ; Untner, J....
Poster presentation at the 23rd annual conference of the American Association of Behavioral and Social Sciences, Las Vegas, NV..
Peer Reviewed
Modeling of acoustic enhanced electrochemical polishing processes.
Landskron, Johannes; Tietze, Sabrina; Wolf, Conrad R.; Drese, Klaus Stefan (2020)
Vortrag auf der Acoustofluidics 2020 2020.
Self-reported treatment expectations and motivations of a large cohort of patients with ankylosing spondylitis (AS), rheumatoid arthritis (RA) and psoriatic arthritis (PA) attending the Gastein Healing Galleries regularly
Offenbächer, M.; Kohls, Niko; Hanshans, C.; Sirois, F. M.; Hirsch, J. K.; Untner, J.; Hölzl, B.; Gaisberger, M.; Toussaint, L. (2019)
Offenbächer, M.; Kohls, Niko; Hanshans, C.; Sirois, F. M.; Hirsch, J. K.; Untner, J....
J Mineralstoffwechsel und Muskuloskelettale Erkrankungen; 4; 125..
Peer Reviewed
Self-reported treatment expectations and motivations of a large cohort of patients with fibromyalgia (FM) and osteoarthritis (OA) attending the Gastein Healing Galleries regularly
Offenbächer, M.; Kohls, Niko; Hanshans, C.; Sirois, F. M.; Hirsch, J. K.; Untner, J.; Hölzl, B.; Gaisberger, M.; Toussaint, L. (2019)
Offenbächer, M.; Kohls, Niko; Hanshans, C.; Sirois, F. M.; Hirsch, J. K.; Untner, J....
J Mineralstoffwechsel und Muskuloskelettale Erkrankungen 2019; 4; 126..
Peer Reviewed
Influence of self-reported treatment expectations and motivations on treatment outcomes of a large cohort of patients with ankylosing spondylitis (AS), rheumatoid arthritis (RA), fibromyalgia (FM) and other chronic pain syndromes (CP) regularly attending the Gastein Healing Gallery
Toussaint, L.; Kohls, Niko; Hanshans, C.; Sirois, F. M.; Hirsch, J. K.; Untner, J.; Hölzl, B.; Gaisberger, M.; Offenbächer, M. (2019)
Toussaint, L.; Kohls, Niko; Hanshans, C.; Sirois, F. M.; Hirsch, J. K.; Untner, J....
J Mineralstoffwechsel und Muskuloskelettale Erkrankungen 2019; 4; 124..
Peer Reviewed
Fakultät Angewandte Naturwissenschaften und Gesundheit (FNG)
Hochschule Coburg
Friedrich-Streib-Str. 2
96450 Coburg
Monika Schnabel
Forschungsreferentin, EU-Referentin
T +49 9561 317 8062
monika.schnabel[at]hs-coburg.de