PhD student | Antje Vondran |
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Duration | 2024-10-14 - 2029-10-13 |
Scientific supervisors HS-Coburg | Prof. Dr. Matthias Noll und Prof. Dr. Stefan Kalkhof |
Institutions |
Hochschule Coburg Promotionszentrum Analytics4Health (A4H) Fakultät Angewandte Naturwissenschaften und Gesundheit (FNG) |
Scientific supervisor (extern) |
The persistence of Listeria monocytogenes presents significant challenges in food pro-duction. Among its survival strategies, the formation of viable-but-non-culturable (VBNC) cells is particularly noteworthy. Understanding the characteristics and progres-sion of these cells is crucial for ensuring food safety. Persistent Listeria monocyto-genes that survives disinfection treatments can lead to recurrent product contamina-tions in food supply chain. Food-borne infections resulting from these, pose a risk to human health. Increased microbial tolerance leads to delayed die-off kinetics without adaptation in the sense of genetic modification. In the context of microbial persistence, Listeria monocytogenes will be characterised with focus on cellular regulatory mecha-nisms.
Keywords: biocide, tolerance, pathogen microorganism, persistence, viable-but-non-culturable (VBNC), Listeria monocytogenes, die-off kinetic, disinfection efficiency, dis-infection strategy, growth phase, metabolic activity, dormancy, survival, adaptation, resistance, isobaric tagging for relative or absolute quantification (iTRAQ), tandem mass tags (TMT), Listeria proteome, flow cytometry