The Challenges of Listeria monocytogenes Persistence and viable-but-non-culturable cells (VBNC) in Food Production

PhD student Antje Vondran
Duration 2024-10-14 - 2029-10-13
Scientific supervisors HS-Coburg Prof. Dr. Matthias Noll und Prof. Dr. Stefan Kalkhof
Institutions Hochschule Coburg
Promotionszentrum Analytics4Health (A4H)
Fakultät Angewandte Naturwissenschaften und Gesundheit (FNG)
Scientific supervisor (extern)

Abstract

The persistence of Listeria monocytogenes presents significant challenges in food pro-duction. Among its survival strategies, the formation of viable-but-non-culturable (VBNC) cells is particularly noteworthy. Understanding the characteristics and progres-sion of these cells is crucial for ensuring food safety. Persistent Listeria monocyto-genes that survives disinfection treatments can lead to recurrent product contamina-tions in food supply chain. Food-borne infections resulting from these, pose a risk to human health. Increased microbial tolerance leads to delayed die-off kinetics without adaptation in the sense of genetic modification. In the context of microbial persistence, Listeria monocytogenes will be characterised with focus on cellular regulatory mecha-nisms.

Keywords: biocide, tolerance, pathogen microorganism, persistence, viable-but-non-culturable (VBNC), Listeria monocytogenes, die-off kinetic, disinfection efficiency, dis-infection strategy, growth phase, metabolic activity, dormancy, survival, adaptation, resistance, isobaric tagging for relative or absolute quantification (iTRAQ), tandem mass tags (TMT), Listeria proteome, flow cytometry