A Novel High-Content Screening Assay Identified Belinostat as Protective in a FSGS-Like Zebrafish Model
Schindler, Maximilian; Siegerist, Florian; Lange, Tim; Simm, Stefan; Bach, Sophia-Marie; Klawitter, Marianne; Gehrig, Jochen; Gul, Sheraz; Endlich, Nicole (2023)
Schindler, Maximilian; Siegerist, Florian; Lange, Tim; Simm, Stefan; Bach, Sophia-Marie...
Journal of the American Society of Nephrology: JASN 34 (12), 1977–1990.
DOI: 10.1681/ASN.0000000000000235
Open Access Peer Reviewed
BACKGROUND: FSGS affects the complex three-dimensional morphology of podocytes, resulting in loss of filtration barrier function and the development of sclerotic lesions. Therapies to treat FSGS are limited, and podocyte-specific drugs are unavailable. To address the need for treatments to delay or stop FSGS progression, researchers are exploring the repurposing of drugs that have been approved by the US Food and Drug Administration (FDA) for other purposes. METHODS: To identify drugs with potential to treat FSGS, we used a specific zebrafish screening strain to combine a high-content screening (HCS) approach with an in vivo model. This zebrafish screening strain expresses nitroreductase and the red fluorescent protein mCherry exclusively in podocytes (providing an indicator for podocyte depletion), as well as a circulating 78 kDa vitamin D-binding enhanced green fluorescent protein fusion protein (as a readout for proteinuria). To produce FSGS-like lesions in the zebrafish, we added 80 µ M metronidazole into the fish water. We used a specific screening microscope in conjunction with advanced image analysis methods to screen a library of 138 drugs and compounds (including some FDA-approved drugs) for podocyte-protective effects. Promising candidates were validated to be suitable for translational studies. RESULTS: After establishing this novel in vivo HCS assay, we identified seven drugs or compounds that were protective in our FSGS-like model. Validation experiments confirmed that the FDA-approved drug belinostat was protective against larval FSGS. Similar pan-histone deacetylase inhibitors also showed potential to reproduce this effect. CONCLUSIONS: Using an FSGS-like zebrafish model, we developed a novel in vivo HCS assay that identified belinostat and related pan-histone deacetylase inhibitors as potential candidates for treating FSGS.
Evaluation of crowdsourced mortality prediction models as a framework for assessing artificial intelligence in medicine
Bergquist, Timothy; Schaffter, Thomas; Yan, Yao; Yu, Thomas; Prosser, Justin; Gao, Jifan; Chen, Guanhua; Charzewski, Łukasz; Nawalany, Zofia; Brugere, Ivan; Retkute, Renata; Prusokas, Alidivinas; Prusokas, Augustinas; Choi, Yonghwa; Lee, Sanghoon; Choe, Junseok; Lee, Inggeol; Kim, Sunkyu; Kang, Jaewoo; Mooney, Sean; Guinney, Justin; Consortium, Patient (2023)
Bergquist, Timothy; Schaffter, Thomas; Yan, Yao; Yu, Thomas; Prosser, Justin...
Journal of the American Medical Informatics Association: JAMIA 31 (1), 35–44.
DOI: 10.1093/jamia/ocad159
Open Access Peer Reviewed
OBJECTIVE: Applications of machine learning in healthcare are of high interest and have the potential to improve patient care. Yet, the real-world accuracy of these models in clinical practice and on different patient subpopulations remains unclear. To address these important questions, we hosted a community challenge to evaluate methods that predict healthcare outcomes. We focused on the prediction of all-cause mortality as the community challenge question. MATERIALS AND METHODS: Using a Model-to-Data framework, 345 registered participants, coalescing into 25 independent teams, spread over 3 continents and 10 countries, generated 25 accurate models all trained on a dataset of over 1.1 million patients and evaluated on patients prospectively collected over a 1-year observation of a large health system. RESULTS: The top performing team achieved a final area under the receiver operator curve of 0.947 (95% CI, 0.942-0.951) and an area under the precision-recall curve of 0.487 (95% CI, 0.458-0.499) on a prospectively collected patient cohort. DISCUSSION: Post hoc analysis after the challenge revealed that models differ in accuracy on subpopulations, delineated by race or gender, even when they are trained on the same data. CONCLUSION: This is the largest community challenge focused on the evaluation of state-of-the-art machine learning methods in a healthcare system performed to date, revealing both opportunities and pitfalls of clinical AI.
Cuticular microbiome composition of Osmia bicornis is different to Apis mellifera honeybees
Thamm, M.; Reiß, Fabienne; Sohl, Leon; Gabel, M.; Noll, Matthias; Scheiner, Ricarda (2023)
MDPI microorganisms 11, 2780.
DOI: 10.3390/microorganisms11112780
Open Access Peer Reviewed
Fachgespräch: Selbst- und Fremdführung durch Achtsamkeit und die Ausbildung von Resilienz. Online-Workshop für das Bayrische Landesamt für Pflege, Ansbach, 22.11.2023
Kohls, Niko (2023)
Achtsamkeit und Nachhaltigkeit: Eine wissenschaftliche Perspektive. Vortag im Rahmen der Climate School, Hochschule Coburg.
Kohls, Niko (2023)
Achtsamkeit und Resilienz: gesund und erfüllt im Lehrerberuf – geht das? Keynote 15. Lehrergesundheitstag Gesunderhaltung im Lehrerberuf – analog und digital, Hochschule Hof, 16.11.2023.
Kohls, Niko (2023)
Sinn & Kohärenz: Die Psychologie als Lehre von Erleben und Verhalten zwischen Theorie und Empirie.
Kohls, Niko (2023)
Placebo responses and effects: Processes, potential, and ethical considerations in clinical care
Kohls, Niko; Leyva, Monica A.; Giordano, James (2023)
Placebo Effects Through the Lens of Translational Research 2023, 251 - 266.
DOI: 10.1093/med/9780197645444.003.0019
Open Access Peer Reviewed
A photoacoustic based measurement system for dual detection of NO2 and CO2 in combustion exhaust gases
Panzardi, Enza; Drese, Klaus Stefan; Mugnaini, Marco; Parri, Lorenzo ; Vignoli, Valerio; Fort, Ada (2023)
Panzardi, Enza; Drese, Klaus Stefan; Mugnaini, Marco; Parri, Lorenzo ; Vignoli, Valerio...
IEEE Transactions on Instrumentation and Measurement 2023.
DOI: 10.1109/TIM.2023.3328691
Peer Reviewed
Evaluating blood oxygen saturation measurements by popular fitness trackers in postoperative patients: A prospective clinical trial.
Helmer, Philipp; Rodemers, Philipp; Hottenrott, Sebastian; Leppich, Robert; Helwich, Maja; Pryss, Rüdiger; Kranke, Peter; Meybohm, Patrick; Winkler, Bernd E; Sammeth, Michael (2023)
Helmer, Philipp; Rodemers, Philipp; Hottenrott, Sebastian; Leppich, Robert...
iScience 2023/26/11, 108155.
DOI: 10.1016/j.isci.2023.108155
Open Access Peer Reviewed
Blood oxygen saturation is an important clinical parameter, especially in postoperative hospitalized patients, monitored in clinical practice by arterial blood gas (ABG) and/or pulse oximetry that both are not suitable for a long-term continuous monitoring of patients during the entire hospital stay, or beyond. Technological advances developed recently for consumer-grade fitness trackers could-at least in theory-help to fill in this gap, but benchmarks on the applicability and accuracy of these technologies in hospitalized patients are currently lacking. We therefore conducted at the postanaesthesia care unit under controlled settings a prospective clinical trial with 201 patients, comparing in total >1,000 oxygen blood saturation measurements by fitness trackers of three brands with the ABG gold standard and with pulse oximetry. Our results suggest that, despite of an overall still tolerable measuring accuracy, comparatively high dropout rates severely limit the possibilities of employing fitness trackers, particularly during the immediate postoperative period of hospitalized patients.
Alzheimer-Demenz - Neue Therapieoptionen?
Funke, Susanne A.; Funke, Susanne A. (2023)
Kongress des TAO-Gesundheitsjahres 2023 / Hochschule Hof.
Akzeptanz digitaler Gesundheitsleistungen bei Älteren in ländlichen Räumen
Esslinger, Adelheid Susanne (2023)
TAO, Hof.
Tree mycorrhizal type regulates leaf and needle microbial communities, affects microbial assembly and co-occurrence network patterns, and influences litter decomposition rates in temperate forest
Tanunchai, Benjawan; Ji, Li; Schroeter , Simon Andreas; Wahdan, S. F. M.; Tongsuk, K.; Hilke , Ines; Gleixner, Gerd; Buscot, F.; Schulze, Ernst-Detlef ; Noll, Matthias; Purahong, W. (2023)
Tanunchai, Benjawan; Ji, Li; Schroeter , Simon Andreas; Wahdan, S. F. M.; Tongsuk, K....
Frontiers Plant Sciences 14, 1239600.
DOI: 10.3389/fpls.2023.1239600
Open Access Peer Reviewed
Physicians’ experiences of treating long COVID with traditional Chinese medicine ‐ a cross‐sectional survey
Kraft, Jana; Hardy, Anne; Baustädter, Verena; Bögel-Witt, Martina; Krassnig, Katharina; Ziegler, Birgit; Meißner, Karin (2023)
Kraft, Jana; Hardy, Anne; Baustädter, Verena; Bögel-Witt, Martina; Krassnig, Katharina...
Vortrag und Posterpräsentation auf 22. Deutschen Kongresses für Versorgungsforschung 2023, 4.-6.10.2023, Berlin.
Peer Reviewed
Usanovich and Nernst colliding: inconsistencies in the all-in-one acid–base concept?
Flechsig, Gerd-Uwe (2023)
Foundations of Chemistry 2023.
DOI: 10.1007/s10698-023-09482-x
Open Access Peer Reviewed
Among the many acid-base concepts, the theory of Usanovich is one of the least known despite the most general scope including almost all chemical reaction types and even redox chemistry. Published 1939 in a Soviet journal in Russian language, it gained little immediate attention, and was later criticized mainly as being too broad in scope. Although several articles recently remembered Usanovich and his acid–base theory, one major inconsistency again was overseen: the electron is put in a row along with anions. Chemical history probably correctly puts this concept aside, also because it added little explanation capabilities beyond the elaborated considerations of the simultaneously published acid–base theory of Gilbert N. Lewis which was later refined by Pearson (hard and soft acids and bases, “HSAB”). A modified version of the core of Usanovich' concept is finally discussed. It combines the classic protic and aprotic acid–base concepts on the foundations of Lewis’ and Pearsons ideas.
Treatment of Long/Post-COVID with Traditional Chinese Medicine (TCM) –a cross-sectional survey of TCM physicians
Hardy, Anne; Kraft, Jana; Baustädter, Verena; Bögel-Witt, Martina; Krassnig, Katharina; Ziegler, Birgit; Meißner, Karin (2023)
Hardy, Anne; Kraft, Jana; Baustädter, Verena; Bögel-Witt, Martina; Krassnig, Katharina...
Poster auf 20. Internationalem TAO Kongress 2023, Graz, Österreich.
Open Access Peer Reviewed
The effects of pilates and open-label placebos on severe primary dysmenorrhea - a randomized controlled pilot study
Ort, Sandra; Waibl, Paula; Stang, Marina; Funke, Susanne A.; Dalkner, Nina; Meißner, Karin (2023)
Ort, Sandra; Waibl, Paula; Stang, Marina; Funke, Susanne A.; Dalkner, Nina...
German Journal of Sports Medicine 74 (4), 151.
Peer Reviewed
Quantification of Moisture in Masonry via AI Evaluated Broadband Radar Reflectometry
Frenzel, Daniel; Blaschke, Oliver; Franzen, Christoph; Brand, Felix; Haas, Franziska; Troi, Alexandra; Drese, Klaus Stefan (2023)
Frenzel, Daniel; Blaschke, Oliver; Franzen, Christoph; Brand, Felix; Haas, Franziska...
Vortrag: Salt Weathering of Buildings and Stone Sculptures Asia 2023, 195-206.
Neue Therapieoptionen für die Alzheimer-Demenz?
Funke, Susanne A. (2023)
Demenzabend im Rahmen des TAO-Themenjahres 2023 - Gesundheit / Alte Kühlhalle Coburg .
Achtsamkeit im Schulalltag: Die Gesundheit der Lehrkräfte im Blick.
Kohls, Niko (2023)
Interview 2013 (3), 10-12.
Fakultät Angewandte Naturwissenschaften und Gesundheit (FNG)
Hochschule Coburg
Friedrich-Streib-Str. 2
96450 Coburg
Monika Schnabel
Forschungsreferentin, EU-Referentin
T +49 9561 317 8062
monika.schnabel[at]hs-coburg.de