Toc75-V/OEP80 is processed during translocation into chloroplasts, and the membrane-embedded form exposes its POTRA domain to the intermembrane space
Gross, Lucia; Spies, Nicole; Simm, Stefan; Schleiff, Enrico (2020)
FEBS open bio 10 (3), 444–454.
DOI: 10.1002/2211-5463.12791
Open Access Peer Reviewed
The insertion of membrane proteins requires proteinaceous complexes in the cytoplasm, the membrane, and the lumen of organelles. Most of the required complexes have been described, while the components for insertion of β-barrel-type proteins into the outer membrane of chloroplasts remain unknown. The same holds true for the signals required for the insertion of β-barrel-type proteins. At present, only the processing of Toc75-III, the β-barrel-type protein of the central chloroplast translocon with an atypical signal, has been explored in detail. However, it has been debated whether Toc75-V/ outer envelope protein 80 (OEP80), a second protein of the same family, contains a signal and undergoes processing. To substantiate the hypothesis that Toc75-V/OEP80 is processed as well, we reinvestigated the processing in a protoplast-based assay as well as in native membranes. Our results confirm the existence of a cleavable segment. By protease protection and pegylation, we observed intermembrane space localization of the soluble N-terminal domain. Thus, Toc75-V contains a cleavable N-terminal signal and exposes its polypeptide transport-associated domains to the intermembrane space of plastids, where it likely interacts with its substrates.
Detection of the level of DNA cross-linking with cisplatin by electrochemical quartz crystal microbalance
Galagedera, S. K. K.; Flechsig, Gerd-Uwe (2020)
Journal of Electroanalytical Chemistry 862, 113992.
DOI: 10.1016/j.jelechem.2020.113992
Peer Reviewed
Biosensors based on DNA self-assembled monolayers (DNA-SAMs) combined with electrochemical transducers have shown great potential to serve an important role in simple, accurate and inexpensive genetic analysis relevant to many fields. Recently we have reported on observing a significant change in the viscoelasticity of such DNA layers immobilized on gold electrodes, upon redox switching of certain metal complexes at a millisecond time scale using an electrochemical quartz crystal microbalance (EQCM). In this study we have performed chronoamperometry on single stranded and double stranded DNA/6-mercapto-1-hexanol (MCH) mixed SAMs on gold electrodes in the presence of hexaammine ruthenium(III) (RuHex) to monitor the DNA modification with cis-diammine dichloroplatinum(II) (cisplatin). Our EQCM studies confirmed that the redox response of electrostatically bound RuHex is capable of providing quantitative information regarding the extent of DNA cross-linking with cisplatin. We believe that this method can be used in studies to test the interactions of cancer chemotherapeutic medications like cisplatin with ss & dsDNA and ultimately be a new technology for rapid screening of such potential drug candidates that are able to penetrate the blood-brain barrier and interact with DNA in order to inhibit cancer cell growth of brain tumor tissue by forming DNA adducts.
Die deutschsprachige Version der Combat Exposure Scale (D-CES) – erste psychometrische Validierung. Wehrmedizinische Monatsschrift.
Zimmermann, Fred; Walach, H.; Schubmann, R.; Kohls, Niko (2020)
Wehrmedizinische Monatsschrift, 64(2), 58-65. 2020 / 64 (2), 58-65.
Peer Reviewed
Benefits of gratitude and self-compassion for sleep in chronic pain: Mediating effects of stress, anxiety, and depression
Altier, H.; Toussaint, L.; Kohls, Niko; Hanshans, Christian ; Sirios , F. ; Untner, J.; Hölzl, B.; Gaisberger, M.; Offenbaecher, M.; Hirsch, J. K. (2020)
Altier, H.; Toussaint, L.; Kohls, Niko; Hanshans, Christian ; Sirios , F. ; Untner, J....
Poster presentation at the 23rd annual conference of the American Association of Behavioral and Social Sciences, Las Vegas, NV..
Peer Reviewed
Modeling of acoustic enhanced electrochemical polishing processes.
Landskron, Johannes; Tietze, Sabrina; Wolf, Conrad R.; Drese, Klaus Stefan (2020)
Vortrag auf der Acoustofluidics 2020 2020.
Self-reported treatment expectations and motivations of a large cohort of patients with ankylosing spondylitis (AS), rheumatoid arthritis (RA) and psoriatic arthritis (PA) attending the Gastein Healing Galleries regularly
Offenbächer, M.; Kohls, Niko; Hanshans, C.; Sirois, F. M.; Hirsch, J. K.; Untner, J.; Hölzl, B.; Gaisberger, M.; Toussaint, L. (2019)
Offenbächer, M.; Kohls, Niko; Hanshans, C.; Sirois, F. M.; Hirsch, J. K.; Untner, J....
J Mineralstoffwechsel und Muskuloskelettale Erkrankungen; 4; 125..
Peer Reviewed
Self-reported treatment expectations and motivations of a large cohort of patients with fibromyalgia (FM) and osteoarthritis (OA) attending the Gastein Healing Galleries regularly
Offenbächer, M.; Kohls, Niko; Hanshans, C.; Sirois, F. M.; Hirsch, J. K.; Untner, J.; Hölzl, B.; Gaisberger, M.; Toussaint, L. (2019)
Offenbächer, M.; Kohls, Niko; Hanshans, C.; Sirois, F. M.; Hirsch, J. K.; Untner, J....
J Mineralstoffwechsel und Muskuloskelettale Erkrankungen 2019; 4; 126..
Peer Reviewed
Influence of self-reported treatment expectations and motivations on treatment outcomes of a large cohort of patients with ankylosing spondylitis (AS), rheumatoid arthritis (RA), fibromyalgia (FM) and other chronic pain syndromes (CP) regularly attending the Gastein Healing Gallery
Toussaint, L.; Kohls, Niko; Hanshans, C.; Sirois, F. M.; Hirsch, J. K.; Untner, J.; Hölzl, B.; Gaisberger, M.; Offenbächer, M. (2019)
Toussaint, L.; Kohls, Niko; Hanshans, C.; Sirois, F. M.; Hirsch, J. K.; Untner, J....
J Mineralstoffwechsel und Muskuloskelettale Erkrankungen 2019; 4; 124..
Peer Reviewed
Effects of self-compassion and self-forgiveness on functional disability in chronic pain: Exploring stress, depression, and sleep as mediators
Altier, H.; Meek, R.; Toussaint, L.; Kohls, Niko; Hanshans, Christian ; Sirois, F.; Untner, J.; Hölzl, B.; Gaisberger, M.; Offenbaecher, M.; Hirsch, J. K. (2019)
Altier, H.; Meek, R.; Toussaint, L.; Kohls, Niko; Hanshans, Christian ; Sirois, F....
Oral presentation at the 66th Annual Convention of the Tennessee Psychological Association, Nashville, Tennessee.
Peer Reviewed
The intracellular distribution of the components of the GET system in vascular plants
Bodensohn, Uwe; Simm, Stefan; Fischer, Ken; Jäschke, Michelle; Groß, Lucia; Kramer, Katharina; Ehmann, Christian; Rensing, Stefan; Ladig, Roman; Schleiff, Enrico (2019)
Bodensohn, Uwe; Simm, Stefan; Fischer, Ken; Jäschke, Michelle; Groß, Lucia...
Biochimica Et Biophysica Acta. Molecular Cell Research 1866 (10), 1650–1662.
DOI: 10.1016/j.bbamcr.2019.06.012
Open Access Peer Reviewed
The guided entry of tail-anchored proteins (GET) pathway facilitates targeting and insertion of tail-anchored proteins into membranes. In plants, such a protein insertion machinery for the endoplasmic reticulum as well as constituents within mitochondrial and chloroplasts were discovered. Previous phylogenetic analysis revealed that Get3 sequences of Embryophyta form two clades representing cytosolic ("a") and organellar ("bc") GET3 homologs, respectively. Cellular fractionation of Arabidopsis thaliana seedlings and usage of the self-assembly GFP system in protoplasts verified the cytosolic (ATGet3a), plastidic (ATGet3b) and mitochondrial (ATGet3c) localization of the different homologs. The identified plant homologs of Get1 and Get4 in A. thaliana are localized in ER and cytosol, respectively, implicating a degree of conservation of the GET pathway in A. thaliana. Transient expression of Get3 homologs of Solanum lycopersicum, Medicago × varia or Physcomitrella patens with the self-assembly GFP technique in homologous and heterologous systems verified that multiple Get3 homologs with differing subcellular localizations are common in plants. Chloroplast localized Get3 homologs were detected in all tested plant systems. In contrast, mitochondrial localized Get3 homologs were not identified in S. lycopersicum, or P. patens, while we confirmed on the example of A. thaliana proteins that mitochondrial localized Get3 proteins are properly targeted in S. lycopersicum as well.
Characterization of aluminium oxid layers with a laser ultrasonic setup using the dispersion of Rayleigh waves
Brand, Felix; Singer, Ferdinand; Drese, Klaus Stefan (2019)
LUS4Metals. Linz, .
Health beliefs, attitudes, and health-related quality of life in persons with fibromyalgia: mediating role of treatment adherence
Rowe, C.; Sirios , F. ; Toussaint, L.; Kohls, Niko; Nöfer, Eberhard; Offenbacher, M.; Hirsch, J. K. (2019)
Rowe, C.; Sirios , F. ; Toussaint, L.; Kohls, Niko; Nöfer, Eberhard; Offenbacher, M....
Psychology, Health & Medicine, 24(8), 962-977. Retrieved from https://doi.org/10.1080/13548506.2019.1576913. doi:10.1080/13548506.2019.1576913, 962-977.
DOI: 10.1080/13548506.2019.1576913
Peer Reviewed
Challenges and Criticisms in the Field of Spirituality, Religiousness, and Health
Walach, H.; Kohls, Niko (2019)
In G. Lucchetti, M. F. Prieto Peres, & R. F. Damiano (Eds.), Spirituality, Religiousness and Health: From Research to Clinical Practice (pp. 33-48)., 33-48.
Das Quartier als Setting zur Förderung sozialer Integration und Gesundheitskompetenz: das kommunale Gesundheitsförderungsprojekt „Gesunde Südstadt“
Röhrich, Christina; Karl, V.; Pfeifer, G.; John, Dennis; Kohls, Niko (2019)
Das Gesundheitswesen, 81(08/09), 712-712. doi:10.1055/s-0039-1694507.
DOI: doi:10.1055/s-0039-1694507
Peer Reviewed
Functional diversification of tomato HsfA1 factors is based on DNA binding domain properties
El-Shershaby, Asmaa; Ullrich, Sarah; Simm, Stefan; Scharf, Klaus-Dieter; Schleiff, Enrico; Fragkostefanakis, Sotirios (2019)
El-Shershaby, Asmaa; Ullrich, Sarah; Simm, Stefan; Scharf, Klaus-Dieter...
Gene 714, 143985.
DOI: 10.1016/j.gene.2019.143985
Open Access Peer Reviewed
In all eukaryotes, the response to heat stress (HS) is dependent on the activity of HS transcription factors (Hsfs). Plants contain a large number of Hsfs, however, only members of the HsfA1 subfamily are considered as master regulators of stress response and thermotolerance. In Solanum lycopersicum, among the four HsfA1 members, only HsfA1a has been proposed to possess a master regulator function. We performed a comparative analysis of HsfA1a, HsfA1b, HsfA1c and HsfA1e at different levels of regulation and function. HsfA1a is constitutively expressed under control and stress conditions, while the other members are induced in specific tissues and stages of HS response. Despite that all members are localized in the nucleus when expressed in protoplasts, only HsfA1a shows a wide range of basal activity on several HS-induced genes. In contrast, HsfA1b, HsfA1c, and HsfA1e show only high activity for specific subsets of genes. Domain swapping mutants between HsfA1a and HsfA1c revealed that the variation in that transcriptional transactivation activity is due to differences in the DNA binding domain (DBD). Specifically, we identified a conserved arginine (R107) residue in the turn of β3 and β4 sheet in the C-terminus of the DBD of HsfA1a that is highly conserved in plant HsfA1 proteins, but is replaced by leucine and cysteine in tomato HsfA1c and HsfA1e, respectively. Although not directly involved in DNA interaction, R107 contributes to DNA binding and consequently the activity of HsfA1a. Thus, we demonstrate that this variation in DBD in part explains the functional diversification of tomato HsfA1 members.
Mindfulness Meditation and Fantasy Relaxation in a Group Setting Leads to a Diminished Sense of Self and an Increased Present Orientation
Kohls, Niko; Esch, Tobias; Gerber, L.; Adrian, L.; Wittmann, M. (2019)
Behavioral Sciences, 9(8), 87. Retrieved from https://www.mdpi.com/2076-328X/9/8/87 .
Peer Reviewed
Co-orthologues of ribosome biogenesis factors in A. thaliana are differentially regulated by transcription factors
Kovacevic, Jelena; Palm, Denise; Jooss, Domink; Bublak, Daniela; Simm, Stefan; Schleiff, Enrico (2019)
Kovacevic, Jelena; Palm, Denise; Jooss, Domink; Bublak, Daniela; Simm, Stefan...
Plant Cell Reports 38 (8), 937–949.
DOI: 10.1007/s00299-019-02416-y
Open Access Peer Reviewed
Different genes coding for one ribosome biogenesis factor are differentially expressed and are likely under the control of distinct transcription factors, which contributes to the regulatory space for ribosome maturation. Maturation of ribosomes including rRNA processing and modification, rRNA folding and ribosome protein association requires the function of many ribosome biogenesis factors (RBFs). Recent studies document plant-specific variations of the generally conserved process of ribosome biogenesis. For instance, distinct rRNA maturation pathways and intermediates have been identified, the existence of plant specific RBFs has been proposed and several RBFs are encoded by multiple genes. The latter in combination with the discussed ribosome heterogeneity points to a possible function of the different proteins representing one RBF in diversification of ribosomal compositions. Such factor-based regulation would require a differential regulation of their expression, may be even controlled by different transcription factors. We analyzed the expression profiles of genes coding for putative RBFs and transcription factors. Most of the genes coding for RBFs are expressed in a comparable manner, while different genes coding for a single RBF are often differentially expressed. Based on a selected set of genes we document a function of the transcription factors AtMYC1, AtMYC2, AtbHLH105 and AtMYB26 on the regulation of different RBFs. Moreover, on the example of the RBFs LSG1 and BRX1, both encoded by two genes, we give a first hint on a differential transcription factor dependence of expression. Consistent with this observation, the phenotypic analysis of RBF mutants suggests a relation between LSG1-1 and BRX1-1 expression and the transcription factor MYC1. In summary, we propose that the multiple genes coding for one RBF are required to enlarge the regulatory space for ribosome biogenesis.
Bewusstsein für Prävention und Gesundheitsförderung – Bedeutung für die Behandlung chronischer Erkrankungen
Röhrich, Christina; Kohls, Niko (2019)
Zeitschrift Herzblick – Das Merlin-AHF Projekt, Herausgeber Herzkind e. V. Sonderausgabe 3/2019.
Perceived stigma and health-related quality of life in the working uninsured: Does thwarted belongingness play a role? Stigma and Health, 4(1), 1-10.
Hirsch, J. K.; Sirios , F. ; Visser, P.; Brooks, B.; Kohls, Niko; Offenbacher, M.; Toussaint, L. (2019)
Hirsch, J. K.; Sirios , F. ; Visser, P.; Brooks, B.; Kohls, Niko; Offenbacher, M....
Perceived stigma and health-related quality of life in the working uninsured: Does thwarted belongingness play a role? Stigma and Health..
Open Access Peer Reviewed
FRI0693 Resilience traits in a large cohort of patients with ankylosing spondylitis (AS), rheumatoid arthritis (RA) and fibromyalgia (FM)
Toussaint, L.; Kohls, Niko; Hanshans, C.; Vallejo, M.; Rivera, J.; Sirois, F.; Hirsch, J. K.; Untner, J.; Hölzl, B.; Gaisberger, M.; Offenbächer, M. (2019)
Toussaint, L.; Kohls, Niko; Hanshans, C.; Vallejo, M.; Rivera, J.; Sirois, F....
Annals of the Rheumatic Diseases, 78(Suppl 2), 1046-1046. Retrieved from https://ard.bmj.com/content/annrheumdis/78/Suppl_2/1046.1.full.pdf. doi:10.1136/annrheumdis-2019-eular.5361 .
Peer Reviewed
Fakultät Angewandte Naturwissenschaften und Gesundheit (FNG)
Hochschule Coburg
Friedrich-Streib-Str. 2
96450 Coburg
Monika Schnabel
Forschungsreferentin, EU-Referentin
T +49 9561 317 8062
monika.schnabel[at]hs-coburg.de