The membrane proteome of male gametophyte in Solanum lycopersicum
Paul, Puneet; Chaturvedi, Palak; Selymesi, Mario; Ghatak, Arindam; Mesihovic, Anida; Scharf, Klaus-Dieter; Weckwerth, Wolfram; Simm, Stefan; Schleiff, Enrico (2016)
Paul, Puneet; Chaturvedi, Palak; Selymesi, Mario; Ghatak, Arindam; Mesihovic, Anida...
Journal of Proteomics 131, 48–60.
DOI: 10.1016/j.jprot.2015.10.009
Open Access Peer Reviewed
Pollen cells possess specialized cellular compartments separated by membranes. Consequently, mature pollen contains proteinaceous factors for inter- and intracellular transport of metabolites or ions to facilitate the upcoming energy exhausting processes - germination and fertilization. Despite the current advancement in the understanding of pollen development little is known about the role and molecular nature of the membrane proteome that participates in functioning and development of male gametophyte. We dissected the membrane proteome of mature pollen from economically important crop Solanum lycopersicum (tomato). Isolated membrane fractions from mature pollen of two tomato cultivars (cv. Moneymaker and cv. Red setter) were subjected to shotgun proteomics (GEL-LC-Orbitrap-MS). The global tomato protein assignment was achieved by mapping the peptides on reference genome (cv. Heinz 1706) and de novo assembled transcriptome based on mRNA sequencing from the respective cultivar. We identified 687 proteins, where 176 were assigned as putative membrane proteins. About 58% of the identified membrane proteins participate in transport processes. In depth analysis revealed proteins corresponding to energy related pathways (Glycolysis and Krebs cycle) as prerequisite for mature pollen, thereby revealing a reliable model of energy reservoir of the male gametophyte. BIOLOGICAL SIGNIFICANCE: Mature pollen plays an indispensable role in plant fertility and crop production. To decipher the functionality of pollen global proteomics studies have been undertaken. However, these datasets are deficient in membrane proteins due to their low abundance and solubility. The work presented here provides a comprehensive investigation of membrane proteome of male gametophyte of an agriculturally important crop plant tomato. The analysis of membrane enriched fractions from two tomato cultivars ensured an effective profiling of the pollen membrane proteome. Particularly proteins of the Krebs cycle or the glycolysis process have been detected and thus a model for the energy dynamics and preparedness of the male gametophyte for the upcoming events - germination and fertilization is provided.
Integration von Auslandsmodulen in Curricula als Problem der Studiengangs-organisation
Lohrenscheit , Claudia (2015)
Vortrag im Rahmen der DAAD-Jahreskonferenz, Berlin, 1.12.2015 .
Energy Efficiency Solutions for Historic Buildings: A Handbook
Bastian, Zeno (2015)
Open Access
Wohnen als Menschenrecht
Lohrenscheit , Claudia (2015)
Vortrag im Rahmen der Podiumsdiskussion des AK Armut, Stadt Coburg; 10.11.2015.
Recht und Rechtsverwirklichung – zwei launische Forellen und ihre Qualitäten. In: Krahl/ Löffl (Hrsg.): Zwischen den Welten, Bd. 3: Qualitäten.
Buchholz-Schuster, Eckardt (2015)
Recht und Rechtsverwirklichung – zwei launische Forellen und ihre Qualitäten. In: Krahl/ Löffl (Hrsg.): Zwischen den Welten, Bd. 3: Qualitäten, Göttingen 2015, S. 11 ff..
Menschenrechte für Flüchtlinge
Lohrenscheit , Claudia (2015)
Eröffnungsvortrag der Ausstellung „anders – cool“ der Jugendmigrationsdienste; JUZ Bamberg, 27.10.2015.
Das Menschenrecht auf Gesundheit
Lohrenscheit , Claudia (2015)
Vortrag am 26.10.15 im Rahmen der Veranstaltung "Gesundheit ist Menschenrecht – die gesundheitliche Situation von Flüchtlingen in München" des Referats für Gesundheit und Umwelt der LHM, Gasteig München.
Peacebuilding - Gender - Social Work: International human rights dialogue: celebrating the 100th Anniversary of the Women`s Peace Congress
Franger, G.; Lohrenscheit , Claudia (2015)
Internationale Sozialarbeit / Oldenburg 2015 (12), 10-20.
Peer Reviewed
Psychophysiology of duration estimation in experienced mindfulness meditators and matched controls
Otten, S.; Schötz, E.; Wittmann, M.; Kohls, Niko; Schmidt, S.; Meißner, Karin (2015)
Frontiers in Psychology 2015 / 6, 1215.
DOI: 10.3389/fpsyg.2015.01215
Peer Reviewed
Recent research suggests that bodily signals and interoception are strongly related to our sense of time. Mindfulness meditators train to be aware of their body states and therefore could be more accurate at interval timing. In this study, n = 22 experienced mindfulness meditators and n = 22 matched controls performed both, an acoustic and a visual duration reproduction task of 8, 14, and 20 s intervals, while heart rate and skin conductance were continuously assessed. In addition, participants accomplished a heart beat perception task and two selective attention tasks. Results revealed no differences between meditators and controls with respect to performance in duration reproduction or attentional capacities. Additionally no group difference in heart beat perception scores was found. Across all subjects, correlational analyses revealed several associations between performance in the duration reproduction tasks and psychophysiological changes, the latter being also related to heart beat perception scores. Furthermore, former findings of linearly increasing cardiac periods and decreasing skin conductance levels during the auditory duration estimation task (Meissner and Wittmann, 2011) could be replicated, and these changes could also be observed during a visual duration reproduction task. In contrast to our earlier findings, the heart beat perception test was not related with timing performance. Overall, although experienced meditators did not differ from matched controls with respect to duration reproduction and interoceptive awareness, this study adds significantly to the emerging view that time perception is related to autonomic regulation and awareness of body states.
Die Bilder der Arktis. Zur Wissensgeschichte von Raum und Zeit
Holtorf, Christian (2015)
6. Gothaer Kartenwochen, Forschungsbibliothek Gotha, Eröffnungsvortrag.
Feminismus war gestern!? Von Alphamädchen, Supermodels und Karrierefrauen
Lohrenscheit , Claudia (2015)
Fachvortrag im Rahmen der Veranstaltungsreihe „Gender“ in Stadt und Landkreis Coburg; 8.10.2015.
ESYSDEN: Systemoptimierung der Druckluft-Energieversorgung
Epple, Philipp; Fritsche, Manuel (2015)
Green Factory Bavaria Kolloquium, WiSo Nürnberg, Nürnberg, Deutschland, 30. September - 01. Oktober, 2015.
Das geografische Wissen eines Walfängers
Holtorf, Christian (2015)
Jahrestagung der Deutschen Gesellschaft für Geschichte der Medizin, Naturwissenschaft und Technik (DGGMNT) und der Gesellschaft für Wissenschaftsgeschichte (GWG). (PEER REVIEWED .
Grenzen überschreiten - Zur Bedeutung von praktischem Wissen in der Kartografie
Holtorf, Christian (2015)
Jahrestagung der Deutschen Gesellschaft für Geschichte der Medizin, Naturwissenschaft und Technik (DGGMNT) und der Gesellschaft für Wissenschaftsgeschichte (GWG), (PEER REVIEWED) .
Weiterentwicklung von kompetenzbasierten Prüfungsformen im MINT-Masterstudiengang (Weiterkommen-MINT)
Stubner, B. ; Gast, Stefan; Rost, A.; Reißing, Ralf (2015)
2. HD-MINT-Symposium an der Technischen Hochschule Nürnberg, 24./25.09.2015, Nürnberg.
Peer Reviewed
QIAD assay for quantitating a compound’s efficacy in elimination of toxic Aβ oligomers
Brener, Oleksandr; Dunkelmann, Tina; Gremer, Lothar; van Groen, Thomas; Mirecka, Ewa A.; Kadish, Inga; Willuweit, Antje; Kutzsche, Janine; Jürgens, Dagmar; Rudolph, Stephan; Tusche, Markus; Bongen, Patrick; Pietruszka, Jörg; Oesterhelt, Filipp; Langen, Karl-Josef; Demuth, Hans-Ulrich; Janssen, Arnold; Hoyer, Wolfgang; Funke, Susanne A.; Nagel-Steger, Luitgard; Willbold, Dieter (2015)
Brener, Oleksandr; Dunkelmann, Tina; Gremer, Lothar; van Groen, Thomas; Mirecka, Ewa A....
Scientific Reports 2015 / 5, 13222.
DOI: 10.1038/srep13222
Open Access Peer Reviewed
Strong evidence exists for a central role of amyloid ß-protein (Aß)
oligomers in the pathogenesis of Alzheimer`s disease. We have developed a
fast, reliable and robust in vitro assay, termed QIAD, to quantify the
effect of any compound on the Aß aggregate size distribution. Applying
QIAD, we studied the effect of homotaurine, scyllo-inositol, EGCG, the
benzofuran derivative KMS88009, ZAß3W, the D-enantiomeric peptide D3 and
its tandem version D3D3 on Aß aggregation. The predictive power of the
assay for in vivo efficacy is demonstrated by comparing the oligomer
elimination efficiency of D3 and D3D3 with their treatment effects in
animal models of Alzheimer´s disease.
Fördernde Faktoren für eine Gesundheitsförderung in der Allgemeinarztpraxis – ein fokussierter Review
Offenbächer, M.; Poggenburg, S.; Schelling, J.; Hirsch, J. K.; Sirios , F. ; Toussaint, L.; Esch, Tobias; Nöfer, Eberhard; Kohls, Niko (2015)
Offenbächer, M.; Poggenburg, S.; Schelling, J.; Hirsch, J. K.; Sirios , F. ...
Posterbeitrag bei dem Kongress der Deutschen Gesellschaft für Allgemein- und Familienmedizin, Bozen, 17. – 19. September 2015..
Peer Reviewed
Die Umsetzung der Solvency II-Gruppenaufsicht im deutschen Versicherungsaufsichtsgesetz (VAG): Inwieweit gilt deutsches Recht für die Beaufsichtigung internationaler Versicherungsgruppen?
Kraft, Mirko (2015)
Zeitschrift für die gesamte Versicherungswissenschaft (ZVersWiss) 104 (4), 389-409.
DOI: 10.1007/s12297-015-0311-0
Open Access Peer Reviewed
SWEBOS - The Software Engineering Body of Skills
Sedelmaier, Yvonne; Landes, Dieter (2015)
International Journal of Engineering Pedagogy (iJEP) 5, 1 | 12–19..
DOI: 10.3991/ijep.v5i1.4047
Open Access Peer Reviewed
Social Work as a Human Rights Profession
Lohrenscheit , Claudia (2015)
Vortrag im Rahmen des Seminars “Social Work Sciences” an der University of the Free State, Bloemfontein, South Africa, 1.9.2015.
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