Publikationen HS Coburg

Regulation of two GTPases Toc159 and Toc34 in the translocon of the outer envelope of chloroplasts

Wiesemann, Katharina; Simm, Stefan; Mirus, Oliver; Ladig, Roman; Schleiff, Enrico (2019)

Biochimica Et Biophysica Acta. Proteins and Proteomics 1867 (6), 627–636.
DOI: 10.1016/j.bbapap.2019.01.002

Open Access Peer Reviewed

ABSTRACT

The GTPases Toc159 and Toc34 of the translocon of the outer envelope of chloroplasts (TOC) are involved in recognition and transfer of precursor proteins at the cytosolic face of the organelle. Both proteins engage multiple interactions within the translocon during the translocation process, including dimeric states of their G-domains. The units of the Toc34 homodimer are involved in the recognition of the transit peptide representing the translocation signal of precursor proteins. This substrate recognition is part of the regulation of the GTPase cycle of Toc34. The Toc159 monomer and the Toc34 homodimer recognize the transit peptide of the small subunit of Rubisco at the N- and at the C-terminal region, respectively. Analysis of the transit peptide interaction by crosslinking shows that the heterodimer between both G-domains binds pSSU most efficiently. While substrate recognition by Toc34 homodimer was shown to regulate nucleotide exchange, we provide evidence that the high activation energy of the GTPase Toc159 is lowered by substrate recognition. The nucleotide affinity of Toc34G homodimer and Toc159G monomer are distinct, Toc34G homodimer recognizes GDP and Toc159G GTP with highest affinity. Moreover, the analysis of the nucleotide association rates of the monomeric and dimeric receptor units suggests that the heterodimer has an arrangement distinct from the homodimer of Toc34. Based on the biochemical parameters determined we propose a model for the order of events at the cytosolic side of TOC. The molecular processes described by this hypothesis range from transit peptide recognition to perception of the substrate by the translocation channel.

Mindful Leader Development: How Leaders Experience the Effects of Mindfulness Training on Leader Capabilities

Rupprecht, S.; Falke, P.; Kohls, Niko; Tamdjidi, C.; Wittmann, M.; Kersemaekers, W. (2019)

Frontiers in Psychology, 10(1081). doi:10.3389/fpsyg.2019.01081.
DOI: 10.3389/fpsyg.2019.01081

Peer Reviewed

Experimentelle Betrachtung der Wärmeübertragung durch instationäre freie Konvektion an der vertikalen Platte

Schaub, Michael (2019)

Fortschritt-Berichte VDI, Düsseldorf, 2019. Zugl.: Technische Universität Berlin, Dissertation, 2019. 19 (162).
DOI: 10.14279/depositonce-8706

Open Access

The sizing of energy storages or how valuable is the last step?

Floß, Alexander; Schaub, Michael (2019)

Advances in Building Energy Research 14 (03), 355-371.
DOI: 10.1080/17512549.2019.1588166

Peer Reviewed

Von der Psychologie des Horizontes zu den epistemologischen Horizonten der akademischen Psychologie

Kohls, Niko (2019)

In B. Frischmann & C. Holtorf (Eds.), Über den Horizont - Standorte, Grenzen und Perspektiven (pp. 149 - 164): De Gruyter., 149-164.

Experimental investigation of heat transfer by unsteady natural convection at a vertical flat plate

Schaub, Michael; Kriegel, Martin; Brandt, Stefan (2019)

International Journal of Heat and Mass Transfer 136, 1186-1198.
DOI: 10.1016/j.ijheatmasstransfer.2019.03.089

Peer Reviewed

The repressor and co-activator HsfB1 regulates the major heat stress transcription factors in tomato

Fragkostefanakis, Sotirios; Simm, Stefan; El-Shershaby, Asmaa; Hu, Yangjie...

Plant, Cell & Environment 42 (3), 874–890.
DOI: 10.1111/pce.13434

Open Access Peer Reviewed

ABSTRACT

Plants code for a multitude of heat stress transcription factors (Hsfs). Three of them act as central regulators of heat stress (HS) response in tomato (Solanum lycopersicum). HsfA1a regulates the initial response, and HsfA2 controls acquired thermotolerance. HsfB1 is a transcriptional repressor but can also act as co-activator of HsfA1a. Currently, the mode of action and the relevance of the dual function of HsfB1 remain elusive. We examined this in HsfB1 overexpression or suppression transgenic tomato lines. Proteome analysis revealed that HsfB1 overexpression stimulates the co-activator function of HsfB1 and consequently the accumulation of HS-related proteins under non-stress conditions. Plants with enhanced levels of HsfB1 show aberrant growth and development but enhanced thermotolerance. HsfB1 suppression has no significant effect prior to stress. Upon HS, HsfB1 suppression strongly enhances the induction of heat shock proteins due to the higher activity of other HS-induced Hsfs, resulting in increased thermotolerance compared with wild-type. Thereby, HsfB1 acts as co-activator of HsfA1a for several Hsps, but as a transcriptional repressor on other Hsfs, including HsfA1b and HsfA2. The dual function explains the activation of chaperones to enhance protection and regulate the balance between growth and stress response upon deviations from the homeostatic levels of HsfB1.

Plant-specific ribosome biogenesis factors in Arabidopsis thaliana with essential function in rRNA processing

Palm, Denise; Streit, Deniz; Shanmugam, Thiruvenkadam; Weis, Benjamin; Ruprecht, Maike...

Nucleic Acids Research 47 (4), 1880–1895.
DOI: 10.1093/nar/gky1261

Open Access Peer Reviewed

ABSTRACT

rRNA processing and assembly of ribosomal proteins during maturation of ribosomes involve many ribosome biogenesis factors (RBFs). Recent studies identified differences in the set of RBFs in humans and yeast, and the existence of plant-specific RBFs has been proposed as well. To identify such plant-specific RBFs, we characterized T-DNA insertion mutants of 15 Arabidopsis thaliana genes encoding nuclear proteins with nucleotide binding properties that are not orthologues to yeast or human RBFs. Mutants of nine genes show an altered rRNA processing ranging from inhibition of initial 35S pre-rRNA cleavage to final maturation events like the 6S pre-rRNA processing. These phenotypes led to their annotation as ’involved in rRNA processing’ - IRP. The irp mutants are either lethal or show developmental and stress related phenotypes. We identified IRPs for maturation of the plant-specific precursor 5’-5.8S and one affecting the pathway with ITS2 first cleavage of the 35S pre-rRNA transcript. Moreover, we realized that 5’-5.8S processing is essential, while a mutant causing 6S accumulation shows only a weak phenotype. Thus, we demonstrate the importance of the maturation of the plant-specific precursor 5’-5.8S for plant development as well as the occurrence of an ITS2 first cleavage pathway in fast dividing tissues.

Trying to be perfect in an imperfect world: A person-centred test of perfectionism and health in fibromyalgia patients versus healthy controls

Sirios , F. ; Toussaint, L.; Hirsch, J. K.; Kohls, Niko; Weber, Annemarie...

Personality and Individual Differences, 137, 27-32. doi: https://doi.org/10.1016/j.paid.2018.08.005, 27-32.
DOI: 10.1016/j.paid.2018.08.005

Peer Reviewed

Lab on a Chip

Drese, Klaus Stefan (2019)

Wiener klinisches Magazin 2019 22 (4), 172–177.
DOI: 10.1007/s00740-019-0286-x

Machine Learning in der Qualitätskontrolle

Fairuschin, Viktor; Drese, Klaus Stefan (2019)

Jahresmagazin Mess- und Sensortechnik 2019, 36–38.

Nichtinvasive Detektion von Biofilmen in der Hausinstallation.

Tietze, Sabrina; Lasota, Sandra; Ebert, S.; Landskron, J.; Schwuchow, K....

Verivita Symposium Hygiene + Technik / Wetzlar 2019, 21–27.

Measure particulate matter by yourself: data-quality monitoring in a citizen science project

Benabbas, A.; Geißelbrecht, M.; Nikol, G.; Mahr, L.; Nähr, D.; Steuer, S....

Journal of Sensor and Sensor Systems (J. Sens. Sens. Syst.) 2019 8 (2), 317–328.
DOI: 10.5194/jsss-8-317-2019

Peer Reviewed

Sicherheit statt Freiheit?! - Einsatz freiheitsentziehender Maßnahmen (FEM) in der Pflege von Menschen mit Demenz.

Berzlanovich, A.; Kohls, Niko (2019)

Demenz - Prävention und Therapie / Essen 2019, 439–448.

Rechtsphilosophische Horizonte und die Globalisierung

Buchholz-Schuster, Eckardt (2019)

Über den Horizont / Berlin, Boston 2019 (5), 191–212.
DOI: 10.1515/9783110553291-012

Numerical Verification of the Thermodynamic Determination of the Hydraulic Efficiency of Radial Fans

Epple, Philipp; Fritsche, Manuel; Reinker, F.; Wiesche, S. aus der (2019)

Proceedings of the ASME 2019 International Mechanical Engineering Congress and Exposition IMECE2019-11417 2019.
DOI: 10.1115/IMECE2019-11417

Theoretical and Numerical Analysis of the Pressure Distribution and Discharge Velocity in Flows under inclined Sluice Gates

Epple, Philipp; Steppert, Michael; Malcherek, A.; Fritsche, Manuel (2019)

Proceedings of the ASME - JSME - KSME Joint Fluids Engineering Conference 2019, AJKFLUIDS2019-4613 2019.

Peer Reviewed

Über den Horizont

Frischmann, B.; Holtorf, Christian (2019)

SpatioTemporality / RaumZeitlichkeit / Berlin, Boston 2019 (5).
DOI: 10.1515/9783110553291

Numerical Investigation of the Euler Turbomachinery Equation and Analysis of the Impact of the Impeller Design on the Fan Performance by Optimization Study

Fritsche, Manuel; Epple, Philipp; Gast, Stefan; Delgado, A. (2019)

Proceedings of the ASME 2019 International Mechanical Engineering Congress and Exposition IMECE2019-11417 2019.

CFD-Simulation of Centrifugal Fan Performance Characteristics Using Ideal and Real Gas Models for Air and Organic Fluids

Fritsche, Manuel; Epple, Philipp; Hasselmann, K.; Reinker, F.; Wagner, R....

Proceedings of the ASME - JSME - KSME Joint Fluids Engineering Conference 2019, AJKFLUIDS2019-4613 2019.

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Monika Schnabel
Forschungsreferentin, EU-Referentin
T +49 9561 317 8062
monika.schnabel[at]hs-coburg.de