Systemic factors in young human serum influence in vitro responses of human skin and bone marrow-derived blood cells in a microphysiological co-culture system
Ritter, Johanna; Falckenhayn, Cassandra; Qi, Minyue; Gather, Leonie; Gutjahr, Daniel; Schmidt, Johannes; Simm, Stefan; Kalkhof, Stefan; Hildebrand, Janosch; Bosch, Thomas; Winnefeld, Marc; Grönniger, Elke; Siracusa, Annette (2025)
Ritter, Johanna; Falckenhayn, Cassandra; Qi, Minyue; Gather, Leonie; Gutjahr, Daniel...
Aging (Albany NY) 17 (7), 1784–1809.
Peer Reviewed
Aging is a complex process that significantly contributes to age-related diseases and poses significant challenges for effective interventions, with few holistic anti-aging approaches successfully reversing its signs. Heterochronic parabiosis studies illuminated the potential for rejuvenation through blood-borne factors, yet the specific drivers including underlying mechanisms remain largely unknown and until today insights have not been successfully translated to humans. In this study, we were able to recreate rejuvenation of the human skin via systemic factors using a microphysiological system including a 3D skin model and a 3D bone marrow model. Addition of young human serum in comparison to aged human serum resulted in an improvement of proliferation and a reduction of the biological age as measured by methylation-based age clocks in the skin tissue. Interestingly, this effect was only visible in the presence of bone marrow-derived cells. Further investigation of the bone marrow model revealed changes in the cell population in response to young versus aged human serum treatment. Using proteome analysis, we identified 55 potential systemic rejuvenating proteins produced by bone marrow-derived cells. For seven of these proteins, we were able to verify a rejuvenating effect on human skin cells using hallmarks of aging assays, supporting their role as systemic factors rejuvenating human skin tissue.
Simulation of the biocide distribution in soil using PELMO coupled with COMLEAM
Kalkhof, Stefan; Kiefer, Nadine; Klein, Judith; Rohr, M; Noll, Matthias; Burkhart, Michael; Klein, Michael (2025)
Kalkhof, Stefan; Kiefer, Nadine; Klein, Judith; Rohr, M; Noll, Matthias...
Environmental Science and Pollution Research (Springer Nature Link) Volume 32, 2425-2440.
DOI: 10.1007/s11356-024-35760-y
Open Access Peer Reviewed
Facade eluates affect active and total soil microbiome
Kalkhof, Stefan; Reiß, Fabienne; Kiefer, Nadine; Reiß, Pascal; Noll, Matthias (2025)
Environmental Pollution (Science Direct) Volume 364 (125242), 1.
DOI: 10.1016/j.envpol.2024.125242
Peer Reviewed
Active soil microbial composition and proliferation are directly affected by the presence of biocides from building materials
Kalkhof, Stefan; Reiß, Fabienne; Kiefer, Nadine; Purahong, Witoon; Borken , W.; Noll, Matthias (2024)
Kalkhof, Stefan; Reiß, Fabienne; Kiefer, Nadine; Purahong, Witoon; Borken , W....
Science of the Total Environment 912, 168689.
DOI: 10.1016/j.scitotenv.2023.168689
Peer Reviewed
Eluates from facades at the beginning of their service time affect aquatic and sediment organisms
Kalkhof, Stefan; Kiefer, Nadine; Nichterlein, Moritz; Reiß, Fabienne; Runge, M.; Biermann, U.; Wieland, Thomas; Noll, Matthias (2024)
Kalkhof, Stefan; Kiefer, Nadine; Nichterlein, Moritz; Reiß, Fabienne; Runge, M....
Science of the Total Environment. 906, 167531.
DOI: 10.1016/j.scitotenv.2023.167531
Peer Reviewed
Hydration Status of Geriatric Patients Is Associated with Changes in Plasma Proteome, Especially in Proteins Involved in Coagulation
Wester (geb. Hoen), Laura; Pfeffer, Daniel; Schmidt, Johannes; Johannes, Kraft; Kalkhof, Stefan; Hildebrand, Janosch (2023)
Wester (geb. Hoen), Laura; Pfeffer, Daniel; Schmidt, Johannes; Johannes, Kraft...
Nutrients, 3789.
DOI: 10.3390/nu15173789
Open Access Peer Reviewed
Soil microbial composition and proliferation directly respond to the presence of biocides from building materials
Reiß, Fabienne; Kiefer, Nadine; Kalkhof, Stefan; Noll, Matthias (2023)
SETAC EUROPE 33rd ANNUAL MEETING, 30 APRIL - 4 MAY 2023, DUBLIN.
Measurement and simulation of distribution, degradation, maximal soil concentrations and ecotoxic effects of biocides released from building facades
Kiefer, Nadine; Reiß, Fabienne; Klein, Judith; Klein, Michael; Noll, Matthias; Kalkhof, Stefan (2023)
Kiefer, Nadine; Reiß, Fabienne; Klein, Judith; Klein, Michael; Noll, Matthias...
SETAC EUROPE 33rd ANNUAL MEETING, 30 APRIL - 4 MAY 2023, DUBLIN.
Toxicology of elautes from an early stage facade lifetime
Kiefer, Nadine; Nichterlein, Moritz; Reiß, Fabienne; Runge, Madeleine; Biermann, Ulf; Noll, Matthias; Kalkhof, Stefan (2023)
Kiefer, Nadine; Nichterlein, Moritz; Reiß, Fabienne; Runge, Madeleine; Biermann, Ulf...
SETAC EUROPE 33rd ANNUAL MEETING, 30 APRIL - 4 MAY 2023, DUBLIN.
“Longitudinal Proteomics Screening for Bone Healing Biomarkers in Rat Models”
Kalkhof, Stefan (2023)
Vortrag auf "Annual SYMBoD Meeting", Freiburg.
Integrative tissue and plasma proteomics revealed liquid biopsy-accessible indicative biomarkers for impaired bone fracture healing in type 2 diabetes mellitus
Kalkhof, Stefan (2023)
Fate of a biodegradable plastic in forest soil: Dominant tree species and forest types drive changes in microbial community assembly, influence the composition of plastisphere, and affect poly(butylene succinate co adipate) degradation
Tanunchai, Benjawan; Ji, Li; Schröder, O. (Hg.); Gawol, Susanne J.; Geissler, Andreas ; Wahdan, S. F. M.; Buscot, F.; Kalkhof, Stefan; Schulze, Ernst-Detlef ; Noll, Matthias; Purahong, Witoon (2023)
Tanunchai, Benjawan; Ji, Li; Schröder, O. (Hg.); Gawol, Susanne J.; Geissler, Andreas ...
Science of the Total Environment 873, 162230.
DOI: 10.1016/j.scitotenv.2023.162230
Peer Reviewed
Deep omics profiling of diabetic bone regeneration – Dysregulated molecular mechanisms and implications for personalized PCL-scaffold design
Kalkhof, Stefan (2022)
e:Med Meeting 2022 on Systems Medicine November 28-30, 2022 Heidelberg, DKFZ Communication Center.
Release and distribution of biocides from facades and their ecotoxicological effect on soil organisms
Kalkhof, Stefan; Kiefer, Nadine; Reiß, Fabienne; Nichterlein, Moritz; Noll, Matthias (2022)
Kalkhof, Stefan; Kiefer, Nadine; Reiß, Fabienne; Nichterlein, Moritz...
Vortrag auf Abschlusskolloquium zum Forschungsvorhaben „Entwicklung eines Modells zur Bewertung der Umwelteigenschaften üblicher Putze und Mörtel im Außenbereich“ des Fraunhofer Institut für Bauphysik Holzkirchen.
Osmotic Stress Interferes with DNA Damage Response and H2AX Phosphorylation in Human Keratinocytes
Wester (geb. Hoen), Laura; Rudisch , Christoph ; Wick , Michael ; Indenbirken , Daniela; Grundhoff, Adam; Wegwitz, Florian ; Kalkhof, Stefan; Hildebrand, Janosch (2022)
Wester (geb. Hoen), Laura; Rudisch , Christoph ; Wick , Michael ; Indenbirken , Daniela...
Cells 11 (6), 959.
DOI: 10.3390/cells11060959
Open Access Peer Reviewed
Nitrogen fixing bacteria facilitate microbial biodegradation of a bio-based and biodegradable plastic in soils under ambient and future climatic conditions
Tanunchai, Benjawan; Kalkhof, Stefan; Guliyev , V.; Wahdan, S. F. M.; Krstic, Dennis; Schädler , M.; Geissler, Andreas ; Buscot, F.; Blagodatskaya , E.; Noll, Matthias; Purahong, W. (2022)
Tanunchai, Benjawan; Kalkhof, Stefan; Guliyev , V.; Wahdan, S. F. M.; Krstic, Dennis...
Environmental Science: Processes & Impacts Royal Society of Chemistry 2022 / 24, d1em00426c | 233-241.
DOI: 10.1039/D1EM00426C
Peer Reviewed
Prof. Dr. Stefan Kalkhof
Hochschule Coburg
Fakultät Angewandte Naturwissenschaften und Gesundheit (FNG)
Friedrich-Streib-Str. 2
96450 Coburg
T 09561317210 stefan.kalkhof[at]hs-coburg.de
ORCID iD: 0000-0001-6121-7105