Alternative splicing in tomato pollen in response to heat stress

Abstract

Alternative splicing (AS) is a key control mechanism influencing signal response cascades in different developmental stages and under stress conditions. In this study, we examined heat stress (HS)-induced AS in the heat sensitive pollen tissue of two tomato cultivars. To obtain the entire spectrum of HS-related AS, samples taken directly after HS and after recovery were combined and analysed by RNA-seq. For nearly 9,200 genes per cultivar, we observed at least one AS event under HS. In comparison to control, for one cultivar we observed 76% more genes with intron retention (IR) or exon skipping (ES) under HS. Furthermore, 2,343 genes had at least one transcript with IR or ES accumulated under HS in both cultivars. These genes are involved in biological processes like protein folding, gene expression and heat response. Transcriptome assembly of these genes revealed that most of the alternative spliced transcripts possess truncated coding sequences resulting in partial or total loss of functional domains. Moreover, 141 HS specific and 22 HS repressed transcripts were identified. Further on, we propose AS as layer of stress response regulating constitutively expressed genes under HS by isoform abundance. mehr

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Titel	Alternative splicing in tomato pollen in response to heat stress
Medien	DNA research: an international journal for rapid publication of reports on genes and genomes
Heft	2
Band	24
ISSN	1756-1663
Verfasser	Mario Keller, Yangjie Hu, Anida Mesihovic, Sotirios Fragkostefanakis, Enrico Schleiff, Prof. Dr. Stefan Simm
Seiten	205–217
Veröffentlichungsdatum	4.2017
Zitation	Keller, Mario; Hu, Yangjie; Mesihovic, Anida; Fragkostefanakis, Sotirios; Schleiff, Enrico; Simm, Stefan (2017): Alternative splicing in tomato pollen in response to heat stress. DNA research: an international journal for rapid publication of reports on genes and genomes 24 (2), 205–217. DOI: 10.1093/dnares/dsw051