Effect of thermospermine on expression profiling of different gene using massive analysis of cDNA ends (MACE) and vascular maintenance in Arabidopsis

Abstract

Arabidopsis thaliana polyamine oxidase 5 gene (AtPAO5) functions as a thermospermine (T-Spm) oxidase. Aerial growth of its knock-out mutant (Atpao5-2) was significantly repressed by low dose(s) of T-Spm but not by other polyamines. To figure out the underlying mechanism, massive analysis of 3’-cDNA ends was performed. Low dose of T-Spm treatment modulates more than two fold expression 1,398 genes in WT compared to 3186 genes in Atpao5-2. Cell wall, lipid and secondary metabolisms were dramatically affected in low dose T-Spm-treated Atpao5-2, in comparison to other pathways such as TCA cycle-, amino acid- metabolisms and photosynthesis. The cell wall pectin metabolism, cell wall proteins and degradation process were highly modulated. Intriguingly Fe-deficiency responsive genes and drought stress-induced genes were also up-regulated, suggesting the importance of thermospermi’ne flux on regulation of gene network. Histological observation showed that the vascular system of the joint part between stem and leaves was structurally dissociated, indicating its involvement in vascular maintenance. Endogenous increase in T-Spm and reduction in H2O2 contents were found in mutant grown in T-Spm containing media. The results indicate that T-Spm homeostasis by a fine tuned balance of its synthesis and catabolism is important for maintaining gene regulation network and the vascular system in plants. mehr

Mehr zum Titel

Titel	Effect of thermospermine on expression profiling of different gene using massive analysis of cDNA ends (MACE) and vascular maintenance in Arabidopsis
Medien	Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
Heft	3
Band	27
ISSN	0971-5894
Verfasser	G Sagor, Prof. Dr. Stefan Simm, Dong Kim, Masaru Niitsu, Tomonobu Kusano, Thomas Berberich
Seiten	577–586
Veröffentlichungsdatum	3.2021
Zitation	Sagor, G; Simm, Stefan; Kim, Dong; Niitsu, Masaru; Kusano, Tomonobu; Berberich, Thomas (2021): Effect of thermospermine on expression profiling of different gene using massive analysis of cDNA ends (MACE) and vascular maintenance in Arabidopsis. Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology 27 (3), 577–586. DOI: 10.1007/s12298-021-00967-7