Zyxin is important for the stability and function of podocytes, especially during mechanical stretch

Abstract

Podocyte detachment due to mechanical stress is a common issue in hypertension-induced kidney disease. This study highlights the role of zyxin for podocyte stability and function. We have found that zyxin is significantly up-regulated in podocytes after mechanical stretch and relocalizes from focal adhesions to actin filaments. In zyxin knockout podocytes, we found that the loss of zyxin reduced the expression of vinculin and VASP as well as the expression of matrix proteins, such as fibronectin. This suggests that zyxin is a central player in the translation of mechanical forces in podocytes. In vivo, zyxin is highly up-regulated in patients suffering from diabetic nephropathy and in hypertensive DOCA-salt treated mice. Furthermore, zyxin loss in mice resulted in proteinuria and effacement of podocyte foot processes that was measured by super resolution microscopy. This highlights the essential role of zyxin for podocyte maintenance in vitro and in vivo, especially under mechanical stretch. mehr

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Titel	Zyxin is important for the stability and function of podocytes, especially during mechanical stretch
Medien	Communications Biology
Heft	1
Band	7
ISSN	2399-3642
Verfasser	Felix Kliewe, Florian Siegerist, Elke Hammer, Jaafar Al-Hasani, Theodor Amling, Jonas Hollemann, Maximilian Schindler, Vedran Drenic, Prof. Dr. Stefan Simm, Kerstin Amann, Christoph Daniel, Maja Lindenmeyer, Markus Hecker, Uwe Völker, Nicole Endlich
Seiten	446
Veröffentlichungsdatum	4.2024
Zitation	Kliewe, Felix; Siegerist, Florian; Hammer, Elke; Al-Hasani, Jaafar; Amling, Theodor; Hollemann, Jonas; Schindler, Maximilian; Drenic, Vedran; Simm, Stefan; Amann, Kerstin; Daniel, Christoph; Lindenmeyer, Maja; Hecker, Markus; Völker, Uwe; Endlich, Nicole (2024): Zyxin is important for the stability and function of podocytes, especially during mechanical stretch. Communications Biology 7 (1), 446. DOI: 10.1038/s42003-024-06125-5