Detection of the level of DNA cross-linking with cisplatin by electrochemical quartz crystal microbalance
Abstract
Biosensors based on DNA self-assembled monolayers (DNA-SAMs) combined with electrochemical transducers have shown great potential to serve an important role in simple, accurate and inexpensive genetic analysis relevant to many fields. Recently we have reported on observing a significant change in the viscoelasticity of such DNA layers immobilized on gold electrodes, upon redox switching of certain metal complexes at a millisecond time scale using an electrochemical quartz crystal microbalance (EQCM). In this study we have performed chronoamperometry on single stranded and double stranded DNA/6-mercapto-1-hexanol (MCH) mixed SAMs on gold electrodes in the presence of hexaammine ruthenium(III) (RuHex) to monitor the DNA modification with cis-diammine dichloroplatinum(II) (cisplatin). Our EQCM studies confirmed that the redox response of electrostatically bound RuHex is capable of providing quantitative information regarding the extent of DNA cross-linking with cisplatin. We believe that this method can be used in studies to test the interactions of cancer chemotherapeutic medications like cisplatin with ss & dsDNA and ultimately be a new technology for rapid screening of such potential drug candidates that are able to penetrate the blood-brain barrier and interact with DNA in order to inhibit cancer cell growth of brain tumor tissue by forming DNA adducts.
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| Titel | Detection of the level of DNA cross-linking with cisplatin by electrochemical quartz crystal microbalance |
|---|---|
| Medien | Journal of Electroanalytical Chemistry |
| Verlag | Elsevier |
| Heft | --- |
| Band | 862 |
| ISBN | 1572-6657 |
| Verfasser/Herausgeber | S. K. K. Galagedera, Prof. Dr. Gerd-Uwe Flechsig |
| Seiten | --- |
| Veröffentlichungsdatum | 19.02.2020 |
| Projekttitel | --- |
| Zitation | Galagedera, S. K. K.; Flechsig, Gerd-Uwe (2020): Detection of the level of DNA cross-linking with cisplatin by electrochemical quartz crystal microbalance. Journal of Electroanalytical Chemistry 862, 113992. DOI: 10.1016/j.jelechem.2020.113992 |